血管no神经对颈总动脉血管平滑肌增殖影响-effect of vascular no nerve on proliferation of vascular smooth muscle in common carotid artery.docxVIP

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血管no神经对颈总动脉血管平滑肌增殖影响-effect of vascular no nerve on proliferation of vascular smooth muscle in common carotid artery.docx

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血管no神经对颈总动脉血管平滑肌增殖影响-effect of vascular no nerve on proliferation of vascular smooth muscle in common carotid artery

血管NO神经对颈总动脉血管平滑肌增殖的影响中文摘要EffectsofVascularNitricOxideNervesOnSmoothMuscleCellProliferationoftheCommonCarotidArteryAbstractObjectiveToinvestigatetheroleofvascularnitricoxidergicneuronsinregulatingvascularsmoothmusclecellproliferation,andprovidetheoreticalbasisforpreventingandcuringvascularproliferativediseases.MaterialsandMethodsRetrogradetracingwithFluoro-GoldSettheratinsupineposition,makea3cmincisionattheneck,separatethetissuetoexposetherightcommoncarotidarterysheath,inject1ulof30%FGslowlytothespacebetweenadventitiaandmediabyglassmicropipettesyringe,andstaytheneedleinfor15minutes.Theratsthatsurvivedformorethan1weekwillbeperfusedby4%paraformaldehydefordownstreamexperiments.FeCl3-inducedvascularsmoothmuscleproliferationSettheratinsupineposition,makea3cmincisionattheneck,exposetherightcommoncarotidartery,placea5cmx2cmplasticfilmbelowthecarotidartery,placea2mmx1mmfilterpaperwith1μL2.16mol/LFeCl3overthecarotidartery[1].After20min,takeawaytheplasticfilmandfilterpaper,makeskinsuture.NOSimmunohistochemistryandhematoxylin-eosin(HE)stainingThesectionswith0.01PBSafterwashing,closedwith0.04%hydrogenperoxideinmethanol;normalsheepserumclosed20min;NOSantibody(1:500)at4℃overnight;biotinylatedantibodyat30mintwo;SABCat30min,3DABcolormin;eachstepbetween0.01MPBSrinse3times,mounting,NOSexpressionwasobservedbymicroscope.DifferentgroupsofcommoncarotidarteryweresectionedandstainedwithHE,中文摘要血管NO神经对颈总动脉血管平滑肌增殖的影响observedtheproliferationofvascularsmoothmusclechanges.Results:Fluorogoldretrogradetracingshowedthatthecommoncarotidarterywasprimarilyinnervatedbythesuperiorcervicalganglion.FluorogoldretrogradetracingandNOSimmno-stainingdemonstratedthatnitricoxidergicneuronsexistedincervicalsympatheticganglionandnitricoxidergicneuronscontrolthecarotidartery.HEstainingshowedthatthevascularsmoothmusclestartedtoproliferate1dayaftertheFeCl3treatmentandobviousproliferationswereappearedat5daysafterFeCl3treatment.Comparedwiththephenotypesat5daysafterFeCl3treatment,bothinhibitionofNOSbyLNNAandproceedingsym