新型生物光子学光纤器件分析与应用-analysis and application of new bio-photonics optical fiber devices.docxVIP

摘 要生物学是当今科研的热点之一，随之而产生的是各种类型功能的生物传感器，被广泛 的应用于医疗健康、环境监测、食品安全等各种领域。光学传感器由于其高灵敏度、高分 辨率、传输损耗小、响应速度快、无标记、选择性好等等诸多优点，被广泛的应用到生物 领域，生物光子学器件应运而生。本论文将一些新型的材料及结构应用于传感器中，实现 了一些新型的生物光子学器件，并开展了实验研究及应用研究。本论文首先对基于丝素蛋白的生物光子学器件进行了一些探索。丝素蛋白由于其良好 的生物兼容性以及可调控的生物降解性，被广泛的应用于生物领域。而基于丝素蛋白所制 成的各种光学结构表现出了良好的光学特性，在光学领域也具有着极大的前景。故而本文 首先采用自透析法获得纯再生丝素蛋白水溶液，再由其制作各种不同的光学器件，与现有 的光学系统进行结合，研究其光学特性，并将其应用于生物传感领域。本论文然后对基于微纳光纤及介孔微球结构的生物传感器进行了研究及应用。微纳光 纤，由于其较强的倏逝场，在光纤传感领域得到了广泛的应用；介孔微球，由于其较大的 比表面积，高度统一的孔隙大小等特点，对于增强传感器的灵敏性有着明显的效果。通过 静电吸附作用将微纳光纤与介孔微球进行结合，研制出高灵敏度的生物传感器，并成功的 实现了对 GABA 溶液的浓度检测。最后，对于以上工作进行了简要的回顾，总结实验中的不足以及出现的问题，并对下 一步的实验规划提出了设想，同时也对此类型的生物光子学传感器的研究与应用进行了展 望。 关键词：生物传感器，光学传感器，生物光子学器件，丝素蛋白，微纳光纤，介孔微球I暨南大学硕士学位论文AbstractNowadays, biology is one of the most popular field of scientific research. Therefore different types of biosensors with various functions have been invented. They are widely used in all sorts of fields, such as medical treatment and health, environment monitoring and food security. Optical sensors are widely used in biology field because of their numerous advantages such as high sensitivity, high resolution, low transmission loss, instant reaction, free label and selectivity. In this thesis, some new materials and structures are employed in sensors, which enable us to implement some new types of biophotonics devices for experiment research and applications.Firstly, some research on biophotonics devices based on silk fibroin is reported in this thesis. Silk fibroin is widely used in biology field, as it has good biological compatibility and the regulation of biological degradability. Optical devices based on silk fibroin show good optical properties and is very promising in optical field for various applications. Therefore, in this thesis silk fibroin aqueous solution is obtained by self-dialysis process which is employed to make different optical devices. Then the devices are combined with existing optical system to study their optical property and applications in the biological sensing