hvegf165hbd3双基因修饰bmscs及其对放创复合伤的促愈作用分析-h vegf 165 hbd3 double gene modified bms cs and its effect on wound healing.docx

The establishment of hVEGF165/hBD-3 gene-modified BMSCs and the application of the cells to the healing of combined radiation-wound injury as a countermeasureAbstractWith social progress, more and more concern has been paid to whole body and topical ionizing damage. Ionizing damage may result from nuclear power plant accidents, radiation therapy for malignancy, prolonged fluoroscopy, inhalation of excess radionuclides, and nuclear explosions. There is a coordinated healing process following damage, which involves an interacting network of multiple cells, cytokines and extracellular matrix. During normal wound healing, cytokines or growth factors produced by para- or auto-crine mediate and regulate cascade reactions. In combined radiation-wound injury, the wound surface is refractory to healing, with decrease or dysfunction of repair cells, and reduction in growth factors and collagen synthesis and inflammatory cells. During wound healing, Bone marrow-derived mesenchymal stem cells (BMSCs) migrate to the wound or inflammatory sites, contributing to tissue regeneration and reconstruction, stimulating progenitor cells to proliferate and differentiate, secreting growth factors and remodeling tissues. In addition, BMSCs exert special immunoregulatory and anti-inflammatory actions. Vascular endothelial growth factor (VEGF) functions as a mitogen of endothelial cells, a chemotactic mediator, and a vascular permeability inducer; hence, it plays multiple roles in the wound healing cascade, including angiogenesis, endothelial formation and collagen deposition. Since angiogenesis is critical to wound healing, the use of VEGF alone or in combination with other factors has become a focus of research on wound healing. Persistent topical infection is one of the causes for refractory wound surface. Defensins are rich in positive charges, which act on the cytomembrane to kill a wide spectrum of microorganisms. Due to defensins’ peculiar mechanism of action, there is virtually n