hsamir335与卵巢癌侵袭转移关系的分析-analysis of the relationship between h samir 335 and invasion and metastasis of ovarian cancer.docx

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hsamir335与卵巢癌侵袭转移关系的分析-analysis of the relationship between h samir 335 and invasion and metastasis of ovarian cancer.docx

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hsamir335与卵巢癌侵袭转移关系的分析-analysis of the relationship between h samir 335 and invasion and metastasis of ovarian cancer

目录中英文缩略表···························································································1中文摘要···································································································4ABSTRACT5前言···········································································································6材料和方法·······················································································8结果···································································································27讨论···································································································36参考文献···························································································39综述···········································································································42附录···········································································································55致谢···········································································································57英文缩略词表缩略语ANOVA英文全称Analysisofvariance中文全称方差分析APAmmoniumpersulfate过硫酸铵Bcl-wcDNAB-celllymphoma2-like2ComplementarydeoxyribonucleicB细胞淋巴瘤相关蛋白2互补脱氧核糖核酸DEPCDiethylpryocarbonate焦碳酸二乙酯DMSODimethylsulfoxide二甲基亚砜dNTPDeoxyribonucleosidetriphosphate三磷酸脱氧核糖核苷HRPHorseradishperoxidase辣根过氧化物酶KDKilodalton千道尔顿MMP-2Matrixmetalloproteinase2基质金属蛋白2ODOpticaldensity吸光度PAGEPolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳法PBSPhosphateBufferSaline磷酸盐缓冲溶液PCRPolymerasechainreaction聚合酶链反应PMSFPhenylmethanesulfonylfluoride苯甲基磺酰氟化物PVDFPolyvinylideneFluorideMembrane聚偏二氟乙烯膜RNARibonucleicacid核糖核酸RISCRNA-inducedsilencingcomplexRNA诱导沉默复合物Rpmrevolutionsperminute每分钟转速RTReversetranscription逆转录作用SDSSodiumdodecylsulfate十二烷基硫酸钠SPSSStatisticalPackagefortheSocialSciences社会科学统计软件包TEMEDN,N,N,N-Tetramethyl-1,2-ethanediN,N,N,N-四甲基乙二胺hsa-miR-335与卵巢癌侵袭转移关系的研究中文摘要目的：检测has-miR-335在卵巢肿瘤组织中的表达并且分析其与临床病理的相关性；在细胞水平研究has-miR-335在卵巢癌侵袭转移中的作用机制方法：应用实时定量PCR法检测has-miR-335在17例正常卵巢组织、15例卵巢良性肿瘤组织、8例卵巢交界性肿