试验性蛛网膜下腔出血大鼠COMT表达的变化及意义.docVIP

实验性蛛网膜下腔出血大鼠儿茶酚胺氧位甲基转移酶表达的变化及意义 何朝晖，孙晓川，郭宗铎，朱 炬 (重庆400016，重庆医科大学附属第一医院神经外科) [摘要]　目的　观察实验性SAH后早期大鼠儿茶酚胺氧位甲基转移酶（catechol-O-methyltransferase) mRNA和蛋白表达、血浆儿茶酚胺（catecholamine）含量、基底动脉管径和管壁厚度的变化规律，探讨SAH后早期大鼠COMT表达变化的意义。方法 以视交叉前池单次注血法构建大鼠SAH模型。采用RT-PCR、Western blot法检测大鼠SAH后早期各个时相点（SAH后6、12、24、48、72 h）纹状体组织内COMT mRNA和蛋白表达。采用高效液相色谱法测定大鼠相应时相点血浆CA含量。HE染色后测量大鼠相应时相点基底动脉管径和管壁厚度。 结果　与健康对照组和假手术组比较，SAH组大鼠在建模后6 h见COMT mRNA和蛋白表达开始增高（P＜0.01），12 h时达到高峰（P＜0.01），24 h后开始下降（P＜0.01），48 h时仍高于健康对照组和假手术组相应时相点水平（P＜0.01），至建模后72 h时接近正常水平（P＞0.05）。SAH组大鼠在建模后6 h 即见血浆CA含量升高，24 h时达到峰值，24 h后开始下降，至72 h后仍高于健康对照组和假手术组相应时相点水平。SAH组大鼠在建模后12 h基底动脉的管径明显缩小和管壁明显增厚（P＜0.01），至建模后24h最明显（P＜0.01）并持续至建模后48 h（P＜0.01）；建模后72 h，基底动脉的管径和管壁厚度已接近正常大小（P＞0.05）。 结论　实验性SAH可以诱导SAH后早期大鼠纹状体COMT表达增高；SAH后早期CA含量明显升高并伴有CVS发生；SAH后早期CVS和血浆CA含量增高可能与SAH后早期COMT表达增高不够充分和持久有关。 [关键词] 　COMT；蛛网膜下腔出血；脑血管痉挛；大鼠 The expression of catechol-O-methyltransferase in rats after subarachnoid hemorrhage He Zhaohui, Sun Xiaochuan, Guo Zongduo, Zhu Ju (Department of Neurosurgery,the First Affiliated Hospital of Chongqing Medical University, Chongqing,400016,China) [Abstract]:　Objective To observe the changing pattern of mRNA and protein expression of COMT, CA content in blood plasma, diameter and wall thickness of basilar artery in early period after SAH, and to discuss the significance of COMT expression in early period after SAH. Methods Rat SAH model was estabished by single blood injection to prechiasmatic cistern. RT-PCR and Western Blotting were adopted to detect COMT’s mRNA and protein expression respectively in striatum corpora at 6h, 12h, 24h, 48h, 72h after SAH. CA content in rat’s plasm was determined by high performance liquid chromatography and the diameter and wall thickness of basilar artery in rats were measured after SAH in corresponding time points. Results Compared with the control group, After SAH modeling, COMT expression in rats in SAH group began to rise at 6h, reached peak at 12h, then decreased continually at 24h and 48h, return