bmp2和bmp7基因共转染兔骨髓间充质干细胞构建组织工程骨的实验分析word格式论文.docx

higherlevelofRunx-2,Osx,AKPandCollagenIinmRNAlevel,thanotherfourgroups. GroupcanddhadhigherlevelofRunx-2,Osx,AKP andCollagenIinmRNAlevel,than groupaandb.AndmRNAlevelinday14ofRunx-2,Osx,AKPandCollagenIwereall higherthanthatinday7.14daysaftertransfection,groupehadhigherlevelof Osteocalcin andCollagenIin protein level, thanother fourgroups.TheconcentrationofplateletofPRPwas3.74timesofwholeblood.7daysafterculture, SEM demonstrated that theBMSCs still alivedinthePRPgels.Afterdigestion,the BMSCsadheredtoculturedish1dayafterculture.7dayslater,thecellprolifalatedand kept theshapeofspindleand polygon.ConclusionPreparation ofBMSCs bydirect waywas proper.AdenoviralvectorofBMP2andBMP7couldtransfecttheBMSCsandexpresstheBMP2 and BMP7.AftercotransfectedbybothBMP2andBMP7adenoviralvector,BMSCscouldexpress osteogenesis relatedgenehigherin both mRNAlevel and protein level.Mixation with PRP, BMSCs could still kept alive and proliferation.Keywords BMSCs;Adenovirus; BMP2; BMP7; PRP第二军医大学博士学位论文缩略语表APSAmmoniumPersulfate过硫酸铵BCABicinchoninicAcid二喹啉甲酸BMPBoneMorphogeneticProtein骨形态发生蛋白(B)MSCs(Bone Marrow) MesenchymalStemCells(骨髓)间充质干细胞BSABovine SerumAlbumin牛血清白蛋白CPECytopathic Effect细胞病变效应DAB3, 3’-Diaminobenzidine二氨基联苯胺DAPI4,6-Diamidino-2-Phenylindole4,6-二脒基-2-苯基吲哚DMEM-HGDulbeccos MinimumEssential Medium-High GlucoseDMEM-LGDulbeccos MinimumEssential Medium-LowGlucose达尔伯克(氏)必需基本高糖培养基达尔伯克(氏)必需基本低糖培养基DMSODimethylSulfoxide二甲基亚砜EDTAEthyleneDiamineTetraaceticAcid乙二胺四乙酸FACS/FCMFlowCytometry流式细胞术FBSFetalBovine Serum胎牛血清FITCFluorescein Isothiocyanate异硫氰酸荧光素GFPGreen FluorescenceProtein绿色荧光蛋白HRPHorseradishPeroxidase辣根过氧化物酶IBMX3-Isobutyl-1-Methylxanthine3-异丁基-甲基黄嘌呤胰岛素、转铁蛋白、亚硒酸ITS+LAInsulin,Transferrin, SeleniousAccid+LinoleicAcid＋亚油酸MAPCsMultipotentAdultProgenitorCells多能成体祖细胞MOIMultiplicityofInfection感染复数NBT/BCIPNitroblueTetrazolium/5-Bromo-4-Chloro-3-Indolylphosphate四唑氮蓝/5-溴-4-氯-3-吲哚-磷酸Opti-MEMIReduced SerumMediummodification of MEM(Eagles)低血清基础培养基ORFOpenReading Frame开放读码框架-8 Polyacrylamide GelElectropher