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bapta am诱导人卵巢癌细胞a2780凋亡及对grp78表达的影响word格式论文.docx

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bapta am诱导人卵巢癌细胞a2780凋亡及对grp78表达的影响word格式论文

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于必威体育官网网址□ ，在年解密后适用本授权书。不必威体育官网网址□。（请在以上方框内打“√” ）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录英文缩略词表··························································································1中文摘要·································································································· 2Abstract4实验正文部分··························································································6第一章前言························································································6第二章材料与方法··············································································8第三章实验结果················································································17第四章实验讨论················································································22第五章实验结论················································································25参考文献································································································26文献综述································································································29参考文献································································································ 36致谢······································································································42英文缩略词表BAPTA-AMBAPTA-AM钙离子螯合剂GRPsglucose-regulatedproteins葡萄糖调节蛋白GRP78Glucoseregulatedprotein78葡萄糖调节蛋白78GRP94Glucoseregulatedprotein94葡萄糖调节蛋白94IFimmunofluorescence免疫荧光ERendoplasticreticulum内质网TXTritonX-100曲拉通X-100PBSphosphatebuffersaline磷酸盐缓冲液dNTPdeoxyribonucleotidetriphosphate脱氧核糖核苷三磷酸HSPheatshockprotein热休克蛋白SPstressprotein应激蛋白BIPimmunoglobulinheavychainbindingprotein免疫球蛋白重链结合蛋白cDNAcomplementarydeoxyribonucleicacid互补脱氧核糖核酸PCRpolymerasechai