b7h3在急性胰腺炎大鼠血清中表达及意义word格式论文.docxVIP

B7-H3在急性胰腺炎大鼠血清中的表达及意义中文摘要B7-H3 在急性胰腺炎大鼠血清中的表达及意义中文摘要目的观察急性胰腺炎（acutepancreatitis,AP）大鼠血清B7-H3表达水平的变化及其与肿瘤坏死因子-?（tumornecrosisfactor-?,TNF-?）、基质金属蛋白酶-9（matrixmetalloproteinases-9,MMP-9）、白介素-1（interleukin-1,IL-1）和胰腺病理学评分的关系，探讨其在AP发病过程中的作用及意义。方法健康雄性SD大鼠30只，随机分为5组：对照组，急性胰腺炎模型组（分为AP6h组、AP12h组、AP24h组、AP48h组），每组6只。AP组分别于建模后6h、12h、24h、48h处死，对照组于第2次注射后24h处死。ELISA法测定血清B7-H3、TNF-α、MMP-9和IL-1水平并分析B7-H3表达水平与TNF-α、MMP-9和IL-1表达水平变化的相关性；切取胰腺组织行胰腺病理学评分并分析其与B7-H3表达水平变化的相关性；全自动生化分析仪测定血清淀粉酶水平。结果AP组血清中B7-H3、TNF-α、MMP-9、IL-1和淀粉酶水平及胰腺病理学评分均显著高于对照组（P0.01）。血清B7-H3与TNF-α、MMP-9、IL-1水平和胰腺病理学评分变化均呈显著正相关（r=0.969、0.914、0.924、0.952，均P0.01）。结论协同刺激分子B7-H3在AP血清中的表达水平与AP病情的严重程度有关，B7-H3可能通过影响TNF-α、MMP-9和IL-1的产生而参与了AP的炎症反应过程。关键词急性胰腺炎；B7-H3；肿瘤坏死因子-α；基质金属蛋白酶-9；白细胞介素-1作者：徐之静指导老师：汪良芝AbstractChangesofB7-H3LevelsinSerumofRatswithAcutePancreatitisChangesofB7-H3LevelsinSerumofRatswithAcutePancreatitisAbstractObjectiveToobservethedynamicchangesintheserumlevelsof B7-H3inratswithacutepancreatitis(AP),andtoanalysisthecorrelationbetweenB7-H3andtumornecrosisfactor-?（TNF-?）, matrix metalloproteinases-9（MMP-9）, interleukin-1(IL-1)andpancreatichistologicalscores,andto exploreitsroleandsignificanceinAP.Methods30SDmaleratsweredividedrandomlyinto5groups:controlgroup,acutepancreatitisgroup(AP6h,AP12h,AP24h,AP 48h).Themodelgroupswerekilledaftermodelingof 6h,12h,24h,48h,thecontrolgroupwerekilledafterthesecondinjectionof24h.TheserumB7-H3andTNF-?,MMP-9andIL-1concentrationweredeterminedbyELISA,andthecorrelationbetweentheexpressionlevelofB7-H3andTNF-?,MMP-9andIL-1wereanalyzed.Pancreaticsectionswerestainedwithhematoxylinandeosinforhistologicalanalysisandscoring，andthecorrelationbetweentheexpressionlevelofB7-H3 andpancreatichistologicalscores was analyzed.Serumamylase wasdeterminedbyautomaticbiochemicalanalyzer.Results The levels of B7-H3, TNF-?, MMP-9, IL-1and amylase in serum andhistologicalscoringofAPgroupweresignificantlyhigherthanthoseofcontrolgroup（P0.01）.TheserumconcentrationofB7-H3andTNF-?,MMP-9,IL-1andpancreatichistolo