林龄和季节对沙棘人工林土壤微生物群落结构及酶-中国科技论文在线.PDFVIP

中国科技论文在线 林龄和季节对沙棘人工林土壤微生物群落 结构及酶活性的影响# ** 罗蓉，杨苗，余旋 5 （西北农林科技大学林学院，陕西杨凌 712100 ） 摘要：采用时空互代法，以不同林龄8a （YL ）、13a （ZL ）、18a （CL ）的沙棘人工林为研 究对象，对其不同季节土壤微生物种群结构和酶活性的变化规律进行了检测。结果表明：（1 ） 在旱季和雨季，沙棘人工林磷脂脂肪酸总量、细菌脂肪酸总量、真菌脂肪酸总量均随着林龄 10 的增长而增加，且在成熟林时达到最大值。（2 ）在由撂荒地转变为沙棘人工林后，土壤β- 葡糖苷酶、纤维素酶、脲酶、蛋白酶、磷酸酶活性均随着林龄的增长逐渐增加，并于成熟林 时达到最大值。雨季的土壤酶活性均显著高于旱季。（3 ）土壤微生物群落及其酶活性均与 土壤PH 、全氮、速效磷含量呈显著正相关。（4 ）双因素方差分析结果表明，林龄和季节对 沙棘人工林地土壤微生物群落结构特征和酶活性有显著影响。两个因素的交互作用未对土壤 15 微生物群落结构和酶活性造成显著影响。 关键词：沙棘人工林；土壤养分；微生物群落；季节；植被恢复 中图分类号：S714.3; S718.3 Effect of forest age and season on soil microbial community 20 and enzyme activities under sea-buckthorn plantation LUO Rong, YANG Miao, YU Xuan (College of Forestry, Northwest AF University, Yangling, Shaanxi 712100) Abstract: This study investigated the dynamics of soil microbial community and enzyme activities under sea-buckthorn plantations aged 8 years (YL), 13 years (ZL), 18 years (CL) in two seasons. The 25 results showed that, (1) the amount of total PLFAs, bacterial PLFAs, fungal PLFAs increased continuously with forest age. The highest values were detected in CL. (2) soil enzyme activities showed the same trend with soil microbial communities. (3)the total PLFAs, bacteria PLFAs, and fungal PLFAs exhibited significantly positive correlations with soil pH, total nitrogen, available phosphorus. (4) two-way ANOVAs showed that forest age or season has significant impact on