玫瑰茄多糖对电离辐射所致小鼠损伤的保护作用.docVIP

玫瑰茄多糖对电离辐射损伤小鼠抗氧化应激能力的影响 冯艳群，李 路，田树泰 李 静（吉林大学公共卫生学院，吉林省，长春，130021） [摘要]　目的 研究玫瑰茄多糖（PHSL）对电离辐射小鼠抗氧化应激能力的影响。方法 40只健康昆明小鼠常规喂养5d后，随机分为正常对照组(NC)、辐射对照组(IC)和低剂量PHSL给药组（300mg.kg-1.bw-1）、高剂量PHSL给药组（600mg.kg-1.bw-1），连续给药14d，末次给药后24h，对实验组小鼠进行一次性全身X射线均匀照射，总剂量为5.0Gy，于照射后24h处死，检测各组小鼠肝组织匀浆中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力和丙二醛（MDA）的含量。结果 与IC组比较，低、高剂量HSLP给药组小鼠肝组织匀浆中的CAT、SOD、GSH-Px活力明显升高（p0.05），MDA含量明显降低（p0.05）。结论 PHSL可以增强电离辐射小鼠抗氧化应激的能力。 [关键词]　玫瑰茄多糖；电离辐射；抗氧化应激 [中图分类号] R147 Effect of Polysaccharide of Hibiscus sabdariffa L. on Anti-oxidation Capability in Mice Irradiated by Ionizing Radiation FENG Yan-qun，LI Lu，TIAN Shu-tai，LI Jing(School of Public Health, Jilin University, Changchun, 130021)[Abstract] Objective To research the effect of polysaccharide of hibiscus sabdariffa L. on the capability of anti-oxidation in ionizing irradiated mice.Methods Fourty healthy male mice were randomly divided into normal control group (NC), irradiating control group (IC), low dose of PHSL irradiating group (300mg.kg-1.bw-1), and high dose of PHSL irradiating group(600mg.kg-1.bw-1) (n=10). The mice in PHSL groups were treated with PHSL by intragastric injection for 14 days.The mice in all groups except NC group were irradiated with 5 Gy X-ray on the fifteenth day. All the mice were executed at the 24th hour after radiation.Then examined the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and the content of malondialdehyde (MDA) in the liver tissues of each group mice.Results Compared with IC group, the activity of CAT, SOD,GSH-Px in low and high dose of PHSL irradiating group were increased markedly (P0.05); the contents of MDA in low and high dose of PHSL irradiating groups were decreased significantly (P0.05).Conclusion PHSL can enhance the capability of anti-oxidation in ionizing irradiated mice. [Keywords]polysaccharide of hibiscus sabdariffa L.;ionizing radiation;anti-oxidative stress Corresponding author: Li Jing, Tel:0431 E