Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL精品.pdfVIP

NIH Public Access Author Manuscript Biotechnol J . Author manuscript; available in PMC 2015 September 01. N Published in final edited form as: I H Biotechnol J . 2014 September ; 9(9): 1164–1174. doi:10.1002/biot.201300468. - P A A u Enhanced transient recombinant protein production in CHO t h o r cells through the co-transfection of the product gene with Bcl-xL M a n 1,2 2 2 2,* Matthew P. Zustiak , Lisa Jose , Yueqing Xie , Jianwei Zhu , and Micheal J. u s Betenbaugh1,* c r i 1 p Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North t Charles Street, Baltimore, MD, United States 2Biopharmaceutical Development Program, SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B Frederick MD, United States Abstract N I H Transient gene expression is gaining popularity as a method to rapidly produce recombinant - P proteins in mammalian cells. Although significant improvements have been made, in terms of A expression, more improvements are needed to compete with the yields achievable in stable gene A u expression. Much progress has come from optimization of transfection media and parameters, as t h o well as altering culturing conditions to enhance productivity. Recent studies have included using r M cell lines engineered for apoptosis resistance through the constitutive expression of an anti- a apoptotic protein, Bcl-x . In this study we