ureolytic biomineralization reduces proteus mirabilis biofilm susceptibility to ciprofloxacin.ureolytic生物矿化减少变形杆菌生物膜对环丙沙星的易感精品.pdfVIP

crossmark Ureolytic Biomineralization Reduces Proteus mirabilis Biofilm Susceptibility to Ciprofloxacin Xiaobao Li, Nanxi Lu, Hannah R. Brady, Aaron I. Packman Department of Civil and Environmental Engineering, Northwestern University, Evanston, Illinois, USA Ureolytic biomineralization induced by urease-producing bacteria, particularly Proteus mirabilis, is responsible for the forma- tion of urinary tract calculi and the encrustation of indwelling urinary catheters. Such microbial biofilms are challenging to eradicate and contribute to the persistence of catheter-associated urinary tract infections, but the mechanisms responsible for D o this recalcitrance remain obscure. In this study, we characterized the susceptibility of wild-type (ure ) and urease-negative w (ure) P. mirabilis biofilms to killing by ciprofloxacin. Ure biofilms produced fine biomineral precipitates that were homoge- n l o neously distributed within the biofilm biomass in artificial urine, while ure biofilms did not produce biomineral deposits un- a d der identical growth conditions. Following exposure to ciprofloxacin, ure biofilms showed greater survival (less killing) than e