bionano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genome.bionano个体染色体基因组映射支持物理映射和组装在复杂的精品.pdfVIP

Plant Biotechnology Journal (2016) 14, pp. 1523–1531 doi: 10.1111/pbi.12513 BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes 1 2 2 1 1 1 3 Helena Stankova , Alex R. Hastie , Saki Chan , Jan Vrana , Zuzana Tulpova , Marie Kubalakova , Paul Visendi , 4 5 4,6 6 1 1, Satomi Hayashi , Mingcheng Luo , Jacqueline Batley , David Edwards , Jaroslav Dolezel and Hana Simkova * 1 Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic 2BioNano Genomics, San Diego, CA, USA 3Australian Centre for Plant Functional Genomics, University of Queensland, Brisbane, QLD, Australia 4School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia 5Department of Plant Sciences, University of California, Davis, CA, USA 6School of Plant Biology, University of Western Australia, Crawley, WA, Australia Received 4 September 2015; Summary revised 12 November 2015; The assembly of a reference genome sequence of bread wheat is challenging due to its specific accepted 13 November 2015. features such as the genome size of 17 Gbp, polyploid nature an