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微生物学 双语教学Chapter5 and 6.ppt
Chapter5 Microbial Nutrition Concepts 1. Microorganisms require about 10 elements in large quantities, in part because they are used to construct carbohydrates, lipids, proteins, and nucleic acids. Several other elements are needed in very small amounts and are parts of enzymes and cofactors. 2. All microorganisms can be placed in one of a few nutritional categories on the basis of their requirements for carbon, energy, and hydrogen atoms or electrons. 3. Nutrient molecules frequently cannot cross selectively permeable plasma membranes through passive diffusion. They must be transported by one of three major mechanisms involving the use of membrane carrier proteins. Eucaryotic microorganisms also employ endocytosis for nutrient uptake. 4. Culture media are needed to grow microorganisms in the laboratory and to carry out specialized procedures like microbial identification, water and food analysis, and the isolation of articular microorganisms. Many different media are available for these and other purposes. 5. Pure cultures can be obtained through the use of spread plates, streak plates, or pour plates and are required for the careful study of an individual microbial species. Macronutrients 95% or more of cell dry weight is made up of a few major elements: carbon, oxygen, hydrogen, nitrogen, sulfur, phosphorus, potassium, calcium, magnesium and iron. The first six ( C, H, O, N, P and S) are components of carbonhadrates, lipids, proteins and nucleic acids are needed to grow microorganisms in the laboratory and to carry out specialized procedures like microbial identification, water and food analysis, and the isolation of particular microorganisms. A wide variety of media is available for these and other purposes. can be obtained through the use of spread plates, streak plates, or pour plates and are required for the careful study of an individual microbial species. Questions 1. Throughout history, spices have been used as preservatives and t
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