co-culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells.与巨噬细胞共培养提高单独使用和异位基质细胞的入侵活动.pdfVIP

Received: 5 September 2016   Accepted: 14 December 2016 | DOI: 10.1111/cpr.12330 O R I G I N A L A R T I C L E Co-culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells Rachel W. S. Chan1,2  | Cheuk-Lun Lee1,2  | Ernest H. Y. Ng1,2  | William S. B. Yeung1,2 1Department of Obstetrics and Abstract Gynaecology, The University of Hong Kong, Hong Kong, China Objective: To study the effect on endometrial and endometriotic cells after co- culture 2Centre of Reproduction, Development with macrophages, using clonogenic, invasion and self- renewal assays. of Growth, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China Materials and methods: Peripheral blood samples, endometrium and endometriotic tissues were collected. Autologous macrophages were co- cultured with endometrial Correspondence Rachel W. S. Chan, Department of Obstetrics and endometriotic cells. The number of colony- forming units (CFU), invasiveness and and Gynaecology, The University of Hong self- renewal activity after co- culture with macrophages were determined. The cy- Kong, Hong Kong, China. Email: rwschan@hku.hk tokine level of colony- stimulating factor- 1 (CSF- 1) from macrophages with and with- out endometriosis was compared. Funding information This study was supported through a small Results: Co- culture with macrophages significantly increased the clonogenic and inva- project funding from the University of Hong sion ability of endometriotic stromal cells in vitro. Colony- stimulating factor- 1 (CSF- 1) Kong (201209176