某猪场非典型猪瘟发病情况调查及诊断.docVIP

摘要 本实验的目的是首先病猪临床发病特征、病理变化等临床检测方法对分离的病毒进行电镜观察采病猪器官组织经冰冻切片，进行直接免疫荧光染色检验分离的病毒进行电镜观察，其为有囊膜圆形且核为六角形，与猪瘟病毒的形态类似。进行直接免疫荧光染色检验，于荧光显微镜视野中，可见组织上皮细胞浆内呈现黄色、绿色或黄绿色，细胞核不着色，细胞轮廓分明，为猪瘟病毒感染阳性。从流行病学、临床特征、病理变化等方面对发病猪群进行了初步的检查，怀疑为猪瘟。但由于临床诊断并不能进行确诊，所以本研究将从实验室诊断得出最后的结论。 Abstract The purpose of this study is a small farm in Shanxi Province sudden death of pigs for clinical diagnosis, in order to further confirmed by laboratory diagnosis to draw final conclusions. First, the use of pigs and the incidence of clinical features, pathological changes in the clinical detection method for diagnosis. Observation on the isolated viruses. Organs and tissues of pigs taken by the frozen section, direct immunofluorescence test. Viruses isolated observation, it was enveloped circular and hexagonal core, and similar forms of classical swine fever virus. Direct immunofluorescence test, the fluorescent microscope view, we see that the cytoplasm of epithelial cells in yellow, green or yellow green, the nuclei did not stain, cells chiseled, classical swine fever virus-positive. According to the clinical features, pathology, electron microscopic identification and the use of direct immunofluorescence antibody test, and finally confirmed that the swine infected with swine fever virus.  ?From epidemiology, clinical features, pathological changes such as pigs on the incidence of a preliminary inspection of suspected swine fever. However, clinical diagnosis and can not be confirmed, this research will come to a conclusion laboratory diagnosis. Keywords: classical swine fever virus; diagnosis; immunofluorescence  目 录 摘要 I Abstract II 目录 III 1前言 1 1.1 国内外研究现状 1 1.2 研究内容和目的 2 2 材料与方法 3 2.1 材料 3 2.2 方法 4 3 结果 6 3.1 猪群发病的临床表现 6 3.2 病理变化 6 3.3 电镜检测结果 9 3.4 直接免疫荧光抗体试验 10 4 讨论 11 5 结论 13 参考文献 14 致谢 15 猪瘟(Swine Fever, SF)，又称猪霍乱(Hog cholera, HC)，是由猪瘟病毒引起猪的一种高度接触性传染病。猪是该病的唯一自然宿主，主要特征为急性经过、高热稽留、死亡率高和小血管壁变性引起的广泛出血、梗塞和坏死等变化 自从1833年美国俄亥俄洲首先发现猪瘟以来，世界各地有关猪瘟的报道逐渐增多。猪瘟传染性强、病死率高，常给养猪业造成严重损失。1984年国际兽疫局(OI E)将本病列入A类16种法定传染病