comparative evaluation of mscs from bone marrow and adipose tissue seeded in prp-derived scaffold for cartilage regeneration：从骨髓和脂肪组织接种于prp衍生支架软骨再生的骨髓间充质干细胞的比较评价.pdfVIP

Biomaterials 33 (2012) 7008e7018 Contents lists available at SciVerse ScienceDirect Biomaterials journal homepage: www.elsevier .com/locate/biomaterials Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration Xuetao Xie a, Yang Wang a, Cunju Zhao a, Shangchun Guo a, Shen Liu a, Weitao Jia a, Rocky S. Tuan b, Changqing Zhang a, * a Department of Orthopaedic Surgery, Shanghai Sixth People ’s Hospital, Shanghai Jiaotong University School of Medicine, 600 Yishan Road, Shanghai 200233, China b Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh, 450 Technology Drive, Pittsburgh, PA 15219, USA a r t i c l e i n f o a b s t r a c t Article history: The aims of this study were to (1) determine whether platelet-rich plasma (PRP) could be prepared as Received 29 May 2012 a bioactive scaffold capable of endogenous growth factor release for cartilage repair; (2) compare the Accepted 22 June 2012 chondrogenic differentiation ability of mesenchymal stem cells (MSCs) from bone marrow (BMSC) and Available online 19 July 2012 from adipose (ADSC) seeded within the PRP scaffold; and (3) test the efficacy of ADSC-PRP construct in cartilage regeneration in vivo. In vitro evaluation showed that a 3-dimensional scaffold with a mesh-like Keywords: microstructure was formed from PRP, with the capability of endogenou