合生素对肉仔鸡肠道微生物区系影响效应研究.docVIP

合生素对肉仔鸡肠道微生物区系的影响效应研究 杨会玲1，许尧兴2，周利勇1，高玉鹏 （1 西北农林科技大学动物科技学院，陕西杨凌 712100；2浙江省农科院植物保护与微生物研究所，浙江杭州 310021） 摘要：【目的】研究合生素对肉仔鸡盲肠微生物区系的影响。【方法】选择1日龄肉仔鸡450只，随机分成5个日粮处理，分别为基础日粮、基础日粮+抗生素、基础日粮+益生素、基础日粮+益生元、基础日粮+合生素。每处理3个重复，每个重复30只。在21d、42d每个重复选择4只屠宰，无菌采集盲肠内容物，提取细菌基因组总DNA，PCR扩增16s rDNA，用DGGE方法分析盲肠细菌群落结构的变化，用实时荧光定量PCR检测肠道中总菌、乳酸杆菌、双歧杆菌和大肠杆菌数量。【结果】在21、42日龄，日粮添加合生素比单独应用益生素、益生元或抗生素提高了肉仔鸡的平均日增重和饲料转化率（P0.05），肉仔鸡盲肠DGGE条带数（菌群丰富程度）均高于对照组、抗生素组、益生素组、益生元组；基因测序分析发现肉仔鸡盲肠中特异条带主要是不可培养细菌。合生素能促进盲肠总菌数、双歧杆菌和乳酸杆菌的增殖，抑制大肠杆菌数量。【结论】合生素对肉仔鸡促生长的效用与其对盲肠菌群的调控作用有关，且合生素的效果优于益生素、益生元或抗生素。 关键词：合生素；肉仔鸡；肠道菌群；DGGE；qPCR Effects of Dietary synbiotics on intestinal microbial ecology in broiler chickens Yang Hui-ling1, Xu Yao-xing2, Zhou Li-yong1, Gao Yu-peng1 (1College of Animal Science, Northwest AF university, Yangling, Shaanxi 712100; 2Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021) Abstract:【Objective】This study was carried out to investigate the effects of dietary supple- mentation of synbiotics on intestinal microbial flora ecology in broilers.【Method】Four hundred and fifty 1-d-old “Cobb 48” broiler chicks were randomly assigned to 1 to 5 dietary treatments , each treatments allocated to 3 replicates of 30 broilers. The dietary treatments were 1) control, 2) basal diets supplemented with antibiotics, 3) basal diets supplemented with probiotics, 4) basal diets supplemented with prebiotics, 5) basal diets supplemented with synbiotics. On d 21, 42, four birds from each replicate were randomly selected and killed, cecal contents were aseptically collected, extraction the total genomic DNA, then analyzing the intestinal bacterial community by denaturing gradient gel electrophoresis(DGGE) analysis of universal 16S rDNA after amplication with PCR, detecting the populations of tobal bacteria, Lactobacillus, Bifidobacterium and E.Coil by real-time PCR.【Result】On d 21 and 42, The supplementation of synbiotics increased average daily gain and feed conversi