金雀花总黄酮提取工艺优化及抗氧化性研究.docxVIP

浙江农业学报 Acta Agriculturae Zhejiangensis，2015，27( 2) : 278 － 284http: / / www． zjnyxb． cn杨申明，王波，王振吉，等． 金雀花总黄酮提取工艺优化及抗氧化性研究［J］． 浙江农业学报，2015，27( 2) : 278 － 284．DOI: 10. 3969 / j． issn． 1004 － 1524. 2015. 02. 25金雀花总黄酮提取工艺优化及抗氧化性研究波1 ，王振吉1，2，* ，刘杨申明1 ，王强1( 1 楚雄师范学院 化学与生命科学学院，云南 楚雄 675000; 2 云南省高校应用生物学重点实验室，云南 楚雄 675000)4摘 要: 采用 Al( NO3 ) 3 -NaNO2 比色法测定金雀花总黄酮的含量，通过 L9 ( 3 ) 正交试验优化了总黄酮的提取工艺，并对总黄酮清除羟基自由基和 DPPH 自由基的作用进行了初步研究。结果表明: 最佳提取工艺参数为乙醇体积分数 75% ，提取温度 75℃ ，料液比 1∶ 40 ( g·mL － 1 ) ，提取时间 150 min，在此条件下总黄酮平均含量为 1. 543 mg·g － 1 ; 总黄酮对·OH 和 DPPH·的清除率分别可达 42. 59% 和 60. 40% ，说明该类物质 对·OH 和DPPH·有较好的清除能力。关键词: 金雀花; 总黄酮; 正交试验; 羟基自由基; DPPH 自由基中图分类号: Ｒ 284. 2文献标志码:文章编号: 1004-1524( 2015) 02-0278-07AOptimization of extraction technology of total flavonoids in Parochetus communis and the an-tioxidative effectsYANG Shen-ming1 ，WANG Bo1 ，WANG Zhen-ji1，2，* ，LIU Qiang1( 1 Department of Chemistry and Life Science，Chuxiong Normal University，Chuxiong 675000，China; 2 Yunnan Prov-ince Universities Key Laboratory of Applied Biology，Chuxiong 675000，China)Abstract: In this study，the content of total flavonoids in Parochetus communis was determined using Al ( NO3 ) 3 -4NaNO2 method． The extraction technology of total flavonoids was optimized through L9 ( 3 ) orthogonal experiment．The effect of total flavonoids on scavenging of hydroxyl radicals and DPPH radicals in Parochetus communis was alsostudied． The results showed that the optimum extraction parameters were as follows: The volume fraction of ethanolwas 75% ，extraction temperature was 75℃ ，ratio of solid to liquid was 1 ∶ 40 ( g·mL － 1 ) ，and extraction time was150 min． Under such condition，the average content of total flavonoids was 1. 543 mg·g － 1 ，and the scavenging effectof total flavonoids on ·OH and DPPH· were 42. 59% and 60. 40% respectively，implying such material can effec-tively scavenge ·OH and DPPH·．Key words: Parochetus communis; total flavonoids; orthogonal experiment; hydroxyl free radical; DPPH free radical金雀