分子生物学（双语）3 Methods in Molecular Biology and Genetic Engineering.pptVIP

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分子生物学（双语）3 Methods in Molecular Biology and Genetic Engineering.ppt

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分子生物学（双语）3 Methods in Molecular Biology and Genetic Engineering

FIGURE 29: Transgenes can be selected FIGURE 30: Cre excises the sequence between lox sites Structure from Protein Data Bank: 1OUQ. E. Ennifar, et al., Nucleic Acids Res. 31 (2003): 5449-5460. FIGURE 31: Cre/lox excises a target only when Cre is activated FIGURE 32: A knock-in replaces an endogenous gene with an alternative sequence Chapter 3 Methods in Molecular Biology and Genetic Engineering FIGURE CO: Methods in Molecular Biology and Genetic Engineering ? Nicemonkey/D FIGURE 01: The target of a phosphatase (a) and a nuclease (b). An endonuclease (c) and an exonuclease (d) FIGURE 02: Restriction endonuclease FIGURE 03: A restriction map is a linear sequence of sites separated by defined distances on DNA FIGURE 04: Plasmid transformation FIGURE 05: E. coli colonies on agar plates with ampicillin, IPTG, and the color indicator X-gal FIGURE 06: Several types of cloning vectors are available FIGURE 07: A vector can be used in both yeast and bacteria FIGURE 08: Luciferase graph/Firefly Photo ??Cathy Keifer/D FIGURE 09: A mouse promoter controls tissue-specific expression of lacZ Photo courtesy of Robb Krumlauf, Stowers Institute for Medical Research FIGURE 10A: Fluorescent proteins are powerful research tools Courtesy of Joachim Goedhart, Molecular Cytology, SILS, University of Amsterdam. FIGURE 10B: Fluorescent proteins are powerful research tools Reprinted from Vision Res., vol. 45, T. G. Wensel, et al., Rhodopsin-EGFP knock-ins..., pp. 3445-3453. Copyright 2005, with permission from Elsevier [/science/journal. Photo courtesy of Theodore G. Wensel, Baylor Col FIGURE 11: DNA can be introduced into cells in several ways FIGURE 12: An autoradiogram of a gel prepared from the colonies described in Figure 3.5 FIGURE 13: The fluorescent in situ hybridization (FISH) technique Adapted from an illustration by Darryl Leja, National Human Genome Research Institute (). FIGURE 14a b: DNA sizes can be determined by gel electrophoresis Adapted from an illustration by Mi