rp―hplc法测定不同煎煮时间下藏药三果汤中槲皮素、鞣花酸、柯里拉京的含量变化.docVIP

RP―HPLC法测定不同煎煮时间下藏药三果汤中槲皮素、鞣花酸、柯里拉京的含量变化 　　摘要：目的 测定并分析在不同煎煮时间条件下藏药三果汤中槲皮素、鞣花酸、柯里拉京的含量变化。方法 分别称取10 g配置好的藏药三果汤散于4个烧杯中，各加入纯净水30 mL，料液比为1：3。将烧杯置于垫有石棉网的电磁炉上，用180℃将其煮沸，待药品沸腾后，将4个烧杯转移到温度为80℃的恒温水浴锅中，进行10、20、30、40 min的煎煮。待煎煮时间均完毕后，静置冷却至室温，过滤，微孔滤膜抽滤，用反相高效液相色谱测定槲皮素、鞣花酸、柯里拉京的含量变化。结果 藏药三果汤中的槲皮素、鞣花酸、柯里拉京均在煎煮时间为40 min时含量最高。 结论 日常中药的煎煮一般均煎煮超过30 min，因此本实验以煎煮时间对藏药三果汤的某些有效成分含量的影响做出研究，为藏药三果汤的最佳煎煮时间以及某些有效成分的提取、制备及资源开发利用提供一定的参考。 　　关键词：三果汤；槲皮素；鞣花酸；柯里拉京 　　中图分类号：R284 文献标志码：A 文章编号：1007-2349（2017）02-0076-04 　　【Abstract】Objective： To determine and analyze the content changes of quercetin， ellagic acid and corilagin in Sanguotang of Tibetan medicine in different boiling time. Methods： 10 g of Sanguotang powder was weighed in four beakers and 30 mL of pure water was added in each beaker. The ratio of solid to liquid was 1： 3. The beakers were placed on the induction cookers matted with asbestos net to be boiled at 180℃ until the drugs were boiling. The four beakers were transferred to a temperature of 80℃ constant temperature water bath and decocted for 10 min， 20 min， 30 min and 40 min. After decocting， the drug was statically cooled down to room temperature， filtrated， and filtrated with microporous membrane， and the content of quercetin， ellagic acid and corilagine were determined by RP-HPLC. Results： The contents of quercetin， ellagic acid and corilagin were the highest at the time of 40 min. Conclusion： The decoction of traditional Chinese medicine is generally decocted for more than 30 minutes. The study provides the best decocting time and certain reference for the extraction， preparation and utilization of some effective components of Sanguotang. 　　【Key words】Sanguotang， quercetin， ellagic acid， corilagin 　　藏药三果汤又名哲布松汤，初载于我国藏药药典《四部医典》中，是由3味药材即诃子（Terminalia chebula Retz.）又名?X黎勒，毛诃子（Terminalia bellirica Roxb.），又名毗黎勒和余甘子（Phyllanthus emblica L.），又名庵摩勒组成，《四部医典》记载其：“主治瘟疫、紊乱热症、促使热症成型”[1]。后又收载于95年的藏药《部颁标准》[2]、2008年的《四川省阿坝藏族羌族自治州藏药制剂标准》[3]，具有清热生津、调和气血、化解坏血的功效。现代药理学研究表明，三果汤具有抗氧化[4][5][6]、抗癌