ento―ia涂膜剂经皮给药对小鼠急性不完全性脑缺血的保护作用.docVIP

Ento―Ia涂膜剂经皮给药对小鼠急性不完全性脑缺血的保护作用 　　【摘要】目的：通?^复制小鼠急性不完全性脑缺血模型，研究Ento-Ⅰa涂膜剂对脑缺血的保护作用。 方法：140只小鼠分为2批进行实验，每批70只。将70只小鼠随机分为7组，每组10只，分别为假手术组、Ento-Ⅰa涂膜剂组（3750mg?kg-1、1875mg?kg-1、938mg?kg-1）、生理盐水模型组、基质模型组、尼莫地平组（18mg?kg-1，ig）。Ento-Ⅰa涂膜剂预防给药7 d后，测定小鼠结扎双侧颈总动脉带迷走神经后的存活时间及其脑组织SOD活性、MDA含量、EB含量。 结果：Ento-Ⅰa涂膜剂在剂量为1875 mg?kg-1时，可以明显延长小鼠结扎双侧颈总动脉带迷走神经后的存活时间（P 　　【关键词】Ento-Ia涂膜剂；经皮给药；急性不完全性脑缺血；存活时间；脑保护 　　【中图分类号】R74331【文献标志码】 A【文章编号】1007-8517（2017）02-0047-05 　　The Protect of Ento-Ⅰa Plastic on Acute Incomplete Cerebral Ischemia by Mice 　　ZHU Feng1，2HU Yuan1，2LIU Heng1，2，3XIAO Huai1，2，3YANG Zizhong1，2，3 　　ZHAO Hairong1，2，3 　　ZHANG Chenggui1，2，3HE Miao1，2，3* 　　1Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical RD， Institute of Entomoceutics Research， Dali University， Dali 671000， China；2National-Local Joint Engineering Research Center of Entomoceutics， Dali 671000， China；3Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics， Dali University， Dali 671000， China 　　Abstract：Objective To investigote the protective effect of Ento-Ⅰa Plaster on acute mice incomplete cerebral ischemia. Methods 140 mice were divided into two batches to carry on the experiment， each batch 70. 70 rats were randomly divided into 7 groups， 10 in each group， the sham operation group， the Ento-Ⅰa coating group （3750mg?kg-1、1875mg?kg-1、938mg?kg-1）， the saline model group， the matrix model group， the nimodipine group （18 mg?kg-1， ig）. Ento-Ia plastic was given prophylactically for 5 days， establishment mice acute incomplete cerebral ischemia model by total bilateral carotid artery ligation in mice with vagus nerve， record survival time of mice after ischemia and the determination of mouse brain tissue SOD activity， MDA content and the content of Evans blueResults This research shows that Compared with the model group matrix， Ento-Ia plastic can significantly prolong the survival time of mice after ligation of bilateral common carotid artery with vagus nerve at a dose of 1875 m