微囊藻碳酸酐酶活性在不同环境因素下的调节与适应精选.pdfVIP

26 8 Vol.26,No.8 2006 8 ACTA ECOLOGICA SINICA Aug.,2006 1, 2 1, * 1 1 王山杉 , 刘永定 , 邹永东, 李敦海 (1 , 430072; 2, 510640) :3 , ( Microcystis aeruginosa tz. ) , (Microcystis viridis (A. Br.) Lemm) , (Microcystis wesenbergii( om.) om.) ,573(Microcystis sp. 573) ; pH NP , ,;, 6 ; :; ;; : 1000-0933(2006) 08-2443-06 :Q178 : A Modulation and adaptation of carbonic anhydrase activity in Microcy stis spp. under different environmental factors 1, 2 1, * 1 1 WANG Shan-Shan , LIU Yong-Ding , ZOU Yong-Dong , LI Dun-Hai ( 1.Institute of Hydrobiology , Chinese Academy of Sciences , Wuhan, 430072, China; 2. College of Light Industry and Food Engineering , South China University of echnology , Guangzhou, 510640, China) . Acta Ecologica Sinica , 2006, 26( ) :2443~ 244 . Abstract:The carbonic anhydrase (CA) activitieswere determined inthree cyanobacterial species, namely Microcystis aeruginosa tz, Microcystis viridis(A. Br.) Lemm, Microcystis wesenbergii ( om.) om, which are dominant in a lake (Lake Dianchi) subject to major blooms. In more detailed experiments on M . aeruginosa, the effects of inorganic carbon, pH, temperature, nitrogenphosphorus ratio, glucose, and light intensity on CA activity were also investigated. The results showed that the CA activity of M . aeruginosa was influenced dramatically by the concentration of bicarbonate. Consequently, it is suggested that bicarbonate ionswere the main form of inorganic carbon M . aeruginosa could utilize. Cultures grown in the dark exhibited CA activity 6 times higher than that of cells cultured mixotrophically with the addition of glucose. Features of eutrophic wa