安徽辣椒疫霉菌株遗传多样性的RAPD分析-安徽农业大学学报.PDFVIP

安徽农业大学学报, 2011, 38(2): 287-291 Journal of Anhui Agricultural University [DOI]CNKI: 34－1162/S0938.000 安徽省辣椒疫霉菌株遗传多样性的RAPD分析 李 萍，陈艳芬，高智谋* (安徽农业大学植物保护学院，合肥 230036) 摘 要：通过利用RAPD （Random Amplified Polymorphic DNAs ）随机引物中筛选到的可扩增出清晰条带、主 带明显、稳定的10 条引物，对来自安徽合肥、淮南、和县、潜山、岳西、江苏南京和四川邛崃等县市的发病辣椒 上分离鉴定获得23 个辣椒疫霉菌（Phytophthora capsici ）进行全基因组DNA RAPD 标记遗传多样性分析。选用引 物共标出DNA 指纹图带 113 条，其中多态性条带96 条，多态性检测率为84.96%，表明辣椒疫霉菌具有较丰富的 遗传多样性。根据引物扩增的DNA 指纹图谱，运用UPGMA 法分析，以遗传相似系数0.7 为阈值，供试菌株被划 分为3 个遗传聚类组，除部分相同地理来源的菌株被划分为同一组外，其他不同菌株间的遗传相似性与地理来源无 直接相关性。 关键词：辣椒疫霉；RAPD；DNA 指纹图谱；遗传多样性 中图分类号：S436.418.1 文献标识码：A 文章编号：1672−352X (2011)02−0287−05 Phylogenetic analysis among isolates of Phytophthora capsici in Anhui Province by RAPD LI Ping, CHEN Yan-fen, GAO Zhi-mou (School of Plant Protection, Anhui Agricultural University, Hefei 230036) Abstract: Random amplified polymorphic DNA markers were used to analyse genetic diversity among the 23 isolates of Phytophthora Capsici obtained from the infected peppers collected in Hefei, Huainan, Hexian, Qianshan, Yuexi, Nanjing and Qionglai. 10 random primers were selected. A total of 113 reproducible RAPD fragments were scored, of which 96 (84.96%) were polymorphic. This showed that the polymorphism existed among different isolates. The genetic distance and phylogenetic tree were constructed by using of UPGMA cluster analysis. The analysis indicated that the isolates were classified into three groups according to the similarity coef- ficient of 0.7. Only a few isolates from the same locality were divided into the same group and there was no cor- relation between DNA polymorphism and their geographical origins. Key words: Phytophthora capsici ; RAP