脑神康胶囊对高糖乏氧环境下大鼠海马神经元8-OHdG及相关氧化应激因子表达的影响.docVIP

精品论文 参考文献 脑神康胶囊对高糖乏氧环境下大鼠海马神经元8-OHdG及相关氧化应激因子表达的影响 成伟 刘德山（通讯作者） 乔云 高伟 梁尔顺 吕忠宽 　　（山东大学齐鲁医院中医科 山东 济南 250012） 　　【摘 要】目的：研究高糖乏氧环境下体外培养的大鼠海马神经元8-OHdG、HIF-1alpha;、ROS、p47phox、Nox4因子的表达并探讨脑神康胶囊对神经元的保护作用。方法：实验分为正常对照组、高糖乏氧组、脑神康高、中、低剂量组。Elisa检测8-OHdG、ROS、HIF-1alpha;蛋白浓度，Real-time PCR法检测各组海马神经元HIF-1alpha; mRNA、p47phox mRNA、Nox4 mRNA的表达。结果：与高糖乏氧组比较，脑神康各浓度组8-OHdG、ROS、HIF-1alpha;产生明显降低，HIF-1alpha; mRNA、p47phox mRNA和Nox4 mRNA的表达明显下降，以高剂量组下降最明显(P均﹤0.05)。结论：脑神康可保护高糖乏氧环境下大鼠海马神经元，其机制可能与下调8-OHdG、ROS、HIF-1alpha; mRNA、p47phox mRNA和Nox4 mRNA的表达，减轻氧化应激损伤有关。 　　【关键词】海马神经元；高糖乏氧；氧化应激；脑神康胶囊 　　【中图分类号】R285.5 【文献标识码】A 【文章编号】1004-6194（2015）01-0109-02 　　Effects of Naoshenkang capsule on expression of 8-OHdG and other related factors of rat hippocampal neurons in anoxia and high glucose 　　CHENG Wei, LIU De-shan, QIAO Yun, GAO Wei, LIANG Ershun, LV Zhongkuan 　　(Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China) 　　【Abstract】Objective：To study if the Naoshenkang capsule could influence expression of 8-OHdG、HIF-1alpha;、ROS、p47phox、Nox4 of rat hippocampal neurons in anoxia and high glucose. Methods：The experimental groups was carried out as follows: the normal control group, the high glucose and x/xo group; the large, middle and small dosages of Naoshenkang groups. The proteins and mRNA expression of 8-OHdG、ROS、HIF-1alpha; were detected by the Elisa Kit and RT-PCR. Results：Compared with those of the x/xo group, all above parameters in all of the Naoshenkang groups were lower (Plt;0.05). Conclusion： The Naoshenkang capsule can efficiently reduce the expression of 8-OHdG、ROS、HIF-1alpha;、p47phox、Nox4 mRNA, then finally decrease the oxidative stress injury. 　　【Key words】Hippocampal neurons; Naoshenkang capsule; High glucose; Xanthine/xanthine oxidase; Oxidative stress injury 　　糖尿病脑病主要表现为认知功能障碍和大脑的神经生理及结构改变，是高糖长期作用神经系统的结果。研究表明氧化应激在糖尿病大脑损伤中起到重要作用，主要损害海马神经元造成糖尿病认知功能障碍[1]。8-OHdG是DNA氧化应激损伤的重要标志物[2]。本实验研究高糖乏氧环境下大鼠海马神经元8-OHdG、ROS、HIF-1alp