正交法优化白平菇深层培养基的研究-中国管理科学-吉林农业大学.PDFVIP

DOI :10.13327/j.j jlau.2006.01.006 　28 1 　　　　　　　 Vol.28 No.1　 　2006 2 Journal　of 　Jilin　Agricultural　University Feb.2006　 黄清荣, 丛发滋, 刘新海, 辛晓林, 赵　圆 (烟台师范学院生命科学学院, 烟台264025) 　:采用液体深层培养的方法, 研究了白平菇 碳氮源的利用情况, 并以三因素三水平的正交试验进行 了培养基配方的优化。 结果表明:最适碳源为红糖、乳糖、淀粉, 最适氮源为麦麸、酵母粉、蛋白胨, 最适碳氮比 为20～ 30 ∶1, 最适培养基为红糖1.5%、麦麸3%、硫酸镁0.1%。 :白平菇;碳源;氮源;碳氮比;正交试验 :S567.3　　　:A　　　:1000-5684(2005)05-0018-04 Study on Optimum Culture Medium of Pleurotus ostreatus by Orthogonal Test HUA NG Qing-rong, CONG Fa-zi, LIU Xin-ha i, XIN Xiao-lin, ZHAO Yuan (College of Lif e S cience, Yantai Normal University , Yantai 264025, China ) Abstract:The nutritional utilization under submerged culturing for Pleurotus ostreatus was studied.The culture medium reci e was o timized by the orthogonal test in three factors and three levels.The results showed that brown-sugar, lactose and amylum were the o timum carbon sources, wheat-bran, yeast-ow- der and e tone were the o timum nitrogen sources, and the o timum C/N was from 20 ∶1 to 30 ∶1.The best reci e was brown-sugar 1.5%, wheat-bran 3%and MgSO4 0.1%. Key words:P leurotus ostreatus ;carbon source;nitrogen source;C/N;orthogonal test 　　(P leurotus ostreatus ), 1　 , , 100%, , 1.1　 。 8.7%, 1.1.1　试验菌种　, 3 。8 , 。 , 0.031%, 2 1.1.2　培养基　(1): 。, , 20%、3%、 1.5%、 1%、 0.25%、MgSO 0.1%、KH PO 0.2%、 B 4 2 4 1 。、、、 0.001%。 (2):20%、 0.25%、KH