CE 08毛细管电色谱技术.pptVIP

Capillary Electro- Chromatography 毛细管电色谱技术 Capillary Electro-Chromatography毛细管电色谱 (CEC) Micelle electrokinetic capillary chromatography 胶束电动毛细管色谱 (MEKC) Micro emulsion electrokinetic Chromatography 微乳液电动色谱 (MEEKC) Packed capillary electrokinetic chromatography 填充毛细管电动色谱（PCEC） Micelle electrokinetic capillary chromatography-MEKC 胶束电动毛细管色谱法 表面活性剂 亲水端和疏水端 类型：阴离子、阳离子、两性离子和非离子表面活性剂 胶束的形成 临界胶束浓度， Critical micelle concentration －CMC Micro-emulsion electrokinetic chromatographyMEEKC微乳液电动色谱 另一种形成假性固定相的方法 MEEKC separations MEEKC 保留值与LogP的关系 碱性药物的分离 酸性药物的分离 中性药物的分离 抗生素的分离 止痛药的分离，Analgesics 中性和带电混合物的分离 CEC Columns Two forms Particle packed Monolithic Key considerations Phase selectivity Charged surface Frit formation PCEC在蛋白质组分析中有重要潜力 RP-LC的流动相与MS匹配性好 具有CE的高效率 液相色谱固定相的结构 油分散相作为胶束；表面活性剂使之带电，其他表面活性剂改性，成为一类色谱固定相。效果如何？ The advantage is that (1) avoiding the difficulty in preparing the packed capillary; (2) cost reduced greatly; (3) good separation efficiency. 止痛药的分离，酸碱中性同时分离！ Ceftazidime Cefuroxime Cephaloridine Cephalexin Cephalothin Penicillin G PhOMe penicillin Altria KD J.Chromatogr.A, 844 1999 371-386 Basic drugs - chlorpheniramine Acidic analytes - salicylic acid Neutral solutes - paracetamol Charged and neutrals - guaiphenisin - pseudoephedrine - parabens Miola MF Snowden MJ and Altria KD, J.Pharm.Biomed.Analysis, 18 1998 785-797 Packed Capillary Electrokenetic Chromatography PCEC填充毛细管电动色谱 Unimicrotech TM CEC和CLC中的流型比较 pH solvent PCEC of 16 PAHS, 1.5 um nonporous ODS, capillary 100 um id, 20 cm; 70% acetonitrile in 2 mM TRIS; 55 kV for separation, concentration 10E-6~-8 M 被测组分的带电性质如何？ In term of flow rate, as low as nl/min, nanoflow. MS compatibility is the reason why CEC draw so much attention. Proteomics! Notice-the retention order remains while the time reduced at higher pH due to enhanced EOF. Organic additive change the separation speed, and also change the selectivity as demonstrated in the case of THF. * * 1 = methanol; 2 =