氨基糖苷耐药铜绿假单胞菌特异蛋白的研究.docVIP

精品论文 参考文献 氨基糖苷耐药铜绿假单胞菌特异蛋白的研究 宋敏 丁银环 向成玉 黄丽 泸州医学院附属医院检验科 四川 泸州 646000 【摘要】目的 通过筛选氨基糖苷耐药铜绿假单胞菌的特异蛋白，为研究氨基糖苷耐药铜绿假单胞菌耐药机制奠定基础。方法 收集本院2011年9月-2012年6月临床分离的82株铜绿假单胞菌，并根据药敏情况将菌株分为氨基糖苷耐药组和氨基糖苷敏感组。采用SELDI-TOF MS技术筛选出两组的差异蛋白峰，查阅Swiss-prot蛋白数据库初步鉴定差异蛋白。结果 共筛选4个蛋白峰表达最具差异，质荷比分别为10372.87、9900.02、9101.25、7600.04。结论 氨基糖苷耐药铜绿假单胞菌和氨基糖苷敏感铜绿假单胞菌蛋白表达存在差异，这些蛋白对氨基糖苷耐药机制的研究具有重要的意义。 【关键词】表面增强激光解吸电离飞行时间质谱技术；耐药；铜绿假单胞菌 Application of specific proteins for aminoglycoside-resistant pseudomonas aeruginosa SONG Min，DING Yin-huan，XIANG Cheng-yu，HUANG Li， （Department of Laboratory Medicine，The Affiliated Hospital of Luzhou Medical College，Luzhou 646000，China） 【Abstract】Objective：To screen specific protein for aminoglycoside-resistant pseudomonas aeruginosa，and lay the foundation for the study of resistance mechanisms for aminoglycoside-resistant pseudomonas aeruginosa.Methods：From September 2011 to June 2012，a total of 82 strains of pseudomonas aeruginosa isolated from clinical specimens were divided into aminoglycoside-resistant group and aminoglycoside-sensitive group based on susceptibility test.Different protein peaks were selected by SELDI-TOF MS technology between the tow groups，and preliminary identificated in Swiss-prot protein database.Results：Four protein peaks which were 9900.02、7600.04、9101.25 and 10372.87 were found expressing significantly differentially.Conclusions：Proteins expressed differently between aminoglycoside-resistant group and aminoglycoside-sensitive group.These proteins had important significance for the study of aminoglycoside resistance mechanisms 【Keywords】 Surface enhanced laser desorption/ionization-time of flight mass spectrometry technology；resistance；pseudomonas aeruginosa [中图分类号] R977.1+3 [文献标识码] A [文章编号]1672-5018（2016）01-084-02 铜绿假单胞菌（pseudomonas aeruginosa，PAE）是医院感染的主要病原菌之一。近些年来，随着抗菌药物的广泛使用，由铜绿假单胞菌引起的医院感染比例明显上升。卫生部全国细菌耐药监测网显示，2011年铜绿假单胞菌临床分离率在非发酵菌中位居第一[1]。氨基糖苷耐药铜绿假单胞菌的流行和传播使铜绿假单胞菌的控制变得愈加困难。本研究拟从蛋白组学的角度对本院临床分离的铜绿假单胞菌进行氨基糖苷类修饰酶耐药机制研究，旨在为临床合理使用抗菌药物，