模拟内源性电场对心脏组织学电损伤效应.docVIP

精品论文 参考文献 模拟内源性电场对心脏组织学电损伤效应 李隽 刘兴会 扬帆 张扬 杨永曜 蒋清安（通讯作者） (贵州省人民医院心内科 贵州贵阳 550002) 【摘要】目的 探讨低压稳恒直流电场直接作用于正常家兔心脏对心脏电损伤的影响。方法 家兔开胸后直接在心外膜上，于心尖处室间沟下段安放一对Pt电极，施与（0～6）V /cm稳恒直流电场，监测ECG和正电极处心外膜表面温度，光镜和电镜观察心肌组织学变化。结果 17只家兔，场强＞３.5V /cm 出现各种快速室性心律失常。3.5V/cm和（4.0～4.5）V/cm两种电场作用后1h正电极处温度升高分别为（1.05plusmn;0.81）℃、（1.14plusmn;0.23）℃，二者无统计学差异（Pgt;0.05）。3.5V/cm场强作用1h后心肌仅见变性和炎性细胞浸润，（4.0～4.5）V/cm场强作用后还可见凝固性坏死和异常收缩带。结论 场强 le;3.5 V/cm对心脏电损伤不明显。 【关键词】 直流电 电刺激 电损伤 心脏 【中图分类号】R54 【文献标识码】A 【文章编号】2095-1752（2013）29-0019-04 Endogenous electrical fields simulation causing cardiac electrical injuries in histology LI Jun, LIU XinHui, YANG Fan, ZHANG Yang, YANG YongYao, JIANG Qing-an (Department of Cardiology, Guizhou Provincial Hospital, Guiyang, 550002, China) 【Abstract】 Objective: To investigate the cardiac electrical injuries caused by the applied weak steady DC EFs in rabbits. Methods: A pair of Pt electrodes were parallelly placed on the cardiac apex epicardium at the two sides of the main branch of the left coronary artery in the rabbit hearts by thoracotomy without endotracheal intubation, followed by which a DC EFs range of 0 to 6.0V/cm was applied. The limb-lead ECGs and the epicardial temperature in the anelectrode were monitored, and the myocardial histologic structures were observed under the light microscope and the transmission electron microscope. Results: In 17 rabbits EFs of 0 to 3.5 V/cm failed to cause any ventricular arrhythmia. Electrical stimulation for 1h at 3.5 V/cm and 4.0 or 4.5 V/cm only increased the epicardial temperature in the anelectrode by（1.05plusmn;0.81）℃、（1.14plusmn;0.23）℃ respectively, but having no statistical significance（Pgt;0.05）. Additionally, after electrical stimulation for 1h at 3.5 V/cm there were only inflammatory cell infiltrate and denaturation in the myocardium . Howerever, anomalism contraction bands and focal coagulation necrosis of myocardium were still observed after electrical