High Throughput Dna Sequencing With A Microfabricated 96 Lane Capillary Array Electrophoresis Bioprocessor英文电子书.pdfVIP

High throughput DNA sequencing with a microfabricated 96-lane capillary array electrophoresis bioprocessor † ‡ Brian M. Paegel*, Charles A. Emrich , Gary J. Wedemayer*, James R. Scherer*, and Richard A. Mathies* *Department of Chemistry and †Biophysics Graduate Group, University of California, Berkeley, CA 94720 Communicated by Alexander N. Glazer, University of California, Berkeley, CA, November 14, 2001 (received for review September 10, 2001) High throughput DNA sequencing has been performed by using a group has pioneered the design and construction of microfab- microfabricated 96-channel radial capillary array electrophoresis ricated capillary array electrophoresis devices demonstrating (CAE) microchannel plate detected by a 4-color rotary confocal rectilinear networks of 12 and 48 channels for parallel DNA fluorescence scanner. The microchannel plate features a novel fragment analysis (16, 17). The field was further advanced with injector for uniform sieving matrix loading as well as high resolu- the presentation of a 96-lane radial microchannel plate (MCP) tion, tapered turns that provide an effective separation length of layout together with a rotary confocal scanning detector and its 15.9 cm on a compact 150-mm diameter wafer. Expanded common use for high throughput genotyping (18). buffer chambers for the cathode, anode, and waste reservoirs are The primary challenge in adapting the radial 96-lane MCP for used to simplify electrode addressing and to counteract buffering DNA sequencing is attaining the necessary column lengths (13, capacity depletion arising from the high electrophoretic current. 14). Folding channels to incr