Comparative study of the cell wall composition of broccoli, carrot, and tomato Structural characterization of the extractable pectins and hemicelluloses英文电子书.pdf

Carbohydrate Research 346 (2011) 1105–1111 Contents lists available at ScienceDirect Carbohydrate Research journal homepage: www.else /locate/carres Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses Ken Houben, Ruben P. Jolie, Ilse Fraeye, Ann M. Van Loey, Marc E. Hendrickx ⇑ Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Department of Microbial and Molecular Systems (M2S), Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, PB 2457, B-3001 Leuven, Belgium a r t i c l e i n f o a b s t r a c t Article history: This study delivers a comparison of the pectic and hemicellulosic cell wall polysaccharides between the Received 17 December 2010 commonly used vegetables broccoli (stem and florets separately), carrot, and tomato. Alcohol-insoluble Received in revised form 29 March 2011 residues were prepared from the plant sources and sequentially extracted with water, cyclohexane- Accepted 7 April 2011 trans-1,2-diamine tetra-acetic acid, sodium carbonate, and potassium hydroxide solutions, to obtain indi- Available online 13 April 2011 vidual fractions, each containing polysaccharides bound to the cell wall in a specific manner. Structural characterization of the polysaccharide fractions was conducted using colorimetric and chromatographic Keywords: