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High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.高频胚胎发生以及与龙胆苦苷含量高的中国药用植物麻花艽植株再生。Gentian straminea Maxim. (MahuaQinjiao) is an important Chinese medicinal herb that is rich in secoiridoids. To increase the production of this plant, we have developed an efficient tissue culture system for high-frequency plant regeneration. We have also obtained plants with high secoiridoid monomer production through tissue culture and somatic embryogenesis. We have found that the MB medium is better than the B5 medium for callus induction. Calluses induced from immature seeds are superior to those from hypocotyls or young leaves in regeneration via somatic embryogenesis. We have also demonstrated that 2,4-dichlorophenoxyacetic acid is efficient for both callus induction and embryogenesis, indole-3-acetic acid is suitable for embryogenic callus proliferation, and N^sup 6^-(benzyl)-adenine promotes both embryo development and the accumulation of gentiopicroside in the cultures. Regenerated plants have been selected for high gentiopicroside content. One plant contains 5.82% of gentiopicroside, which is higher than the control plants (1.20-3.73%). The regenerated plants are genetically more stable than the calluses based on both cytological and random amplified polymorphic DNA analyses.高频胚胎发生以及与龙胆苦苷含量高的中国药用植物麻花艽植株再生。秦艽。 （MahuaQinjiao）是一种重要的中草药是在secoiridoids丰富。为了提高这家工厂生产，我们开发了一种用于高频高效组织培养植株再生体系。我们亦得到通过组织培养和体细胞胚胎发生与高secoiridoid单体生产厂。我们发现，MB培养基比B5培养基诱导愈伤组织的更好。利用未成熟种子诱导愈伤组织均优于或从下胚轴再生嫩叶通过体细胞胚胎发生的。我们还表明，2,4二氯苯氧乙酸是有效率的为愈伤组织诱导和胚胎，吲哚-3乙酸是合适的胚性愈伤组织增殖和N^6 ^燮- （苄基）腺嘌呤胚胎发育和促进两在龙胆苦苷的文化积累。再生植株已被选定为龙胆苦苷含量高。一个植物含有龙胆苦苷5.82％，比对照植株（1.20-3.73％）为高。再生植株的基因在两个以上的细胞学和随机扩增多态性DNA分析的基础上老茧稳定。ETHNOPHARMACOLOGY OF MEXICAN ASTERACEAETraditional herbal remedies have increased in popularity in Europe and the United States in recent years but have always been important to people living in rural Mexico and to their Mexican