海巴戟天愈伤组织诱导及细胞悬浮培养试验-广东农业科学.PDFVIP

108 广东农业科学 2014 年第 4 期 海巴戟天愈伤组织诱导及细胞悬浮培养试验 张志强 李永成 海 大学食品学院袁海 海口 570228冤 摘要 以海巴戟天叶脉作为外植体袁在 B5 培养基上诱导出淡黄色尧疏松愈 组织袁通过增殖后接种到液体培 养基中袁建立细胞悬浮培养体系袁并对影响细胞悬浮培养的培养基种类尧接种量尧pH 值尧蔗糖含量等因素进行了研 究遥 结果表明院B5 + 1 mg/L 2,4-D + 0.1 mg/L 6-BA 能诱导出淡黄色尧质地疏松的愈 组织曰较佳愈 组织增殖培养 基为 B5 + 2 mg/L NAA + 0.2 mg/L KT 曰细胞在 B5 + 2 mg/L NAA + 0.2 mg/L KT 培养基中悬浮培养袁接种量 60 g/L 细 胞鲜重冤尧 蔗糖 4% 尧pH 值 5.8尧100 r/min尧27益下暗培养袁 海巴戟天悬浮细胞比生长速率为 0.0836 d-1 袁 总蒽醌含量 367.8 mg/L 袁培养周期 27~30 d 遥 关键词 海巴戟天曰愈 组织曰悬浮培养曰蒽醌 中图分类号院 Q813.1 文献标识码 A 文章编号 1004-874X 2014冤04-0108-05 Study on callus induction and cell suspension culture of Morinda citrifolia ZHANG Zhi-qiang, LI Yong-cheng (College of Food Sc ie nc e , Hainan Univ e rs ity, Haikou 570228, China) A bstract: Faint yellow and loose callus was induced from vein of Morinda c itrifolia on B5 medium. After proliferation, the callus was inoculated in liquid medium and cell suspension lines were established. Medium variety, inoculation densities, pH and sucrose concentration were optimized in this research. The results indicated that, faint yellow and loose callus could be induced on B5 medium by adding 1 mg/L 2,4-D and 0.1 mg/L 6-BA; B5 medium by adding 2 mg/L NAA and 0.2 mg/L KT was the better callus proliferation medium. Cells could grow well w ith 2 mg/L NAA and 0.2 mg/L KT in B5 medium w ith 60 g/L (fresh cell weight) of inoculation densities, 4% sucrose, pH 5.8, 100 r/min, and 27 ~28益 in dark. The specific growth rate wa