藏药雪莲原植物水母雪莲及其混淆种类的ITS序列-天津中草药杂志社.PDFVIP

　Chinese Traditional and Herbal Drugs　2001 32 5 ·443· ·· ITS 1 2 2 刘建全 ,陈之端 ,路安民 ( 1. , 　810001;　2. ,　 100093) 　: 　“” ITS,。　 PCR ABI377。 　, 。 　 ITS。 : ;; ITS; : 282. 5　　　: 　　　: 0253 2670(2001) 05 0443 03 R A Comparison on internal transcribed spacers ( ITS)* sequences of Tibetan medicineSaussurea medusa and its easily confusable species 1 2 2 LIU Jian quan , CHEN Zhi duan , LU An min ( 1. , , 810001, ; 2. 　　 Northw est Plateau Institute of Biology Chinese Academy of Sciences Xining Qinghai China Laboratory of Sytematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China) : 　 , Abstract Object In order to identify the medicine at the molecular level the internal transcribed ( ) . . 　 spacers ITS of Saussurea m ed usa Maxim and its easily confusable species were sequenced Methods The double stranded DNA was amplified using PCR systems 9 600 kits and sequenced on an ABI 377 auto . 　 . mated sequencer from both directions Results The ITS sequences of S med usa of different populations , . . showed no variation but there existed distinct variation between S medusa and its confusable species