NEB--推荐双酶切系统.docVIP

窗体顶端 Select 1st enzyme:? ? ? ? Select 2nd enzyme: ? ??? 窗体底端 Enzyme Cat# Temp Supplied NEBuffer Supplements % Activity in NEBuffer BSA SAM 1 2 3 4 BamHI* R0136 37°C NEBuffer 3 Yes No 75 100 100 100 KpnI* R0142 37°C NEBuffer 1 Yes No 100 75 0 50 Double Digest Recommendation(s) for BamHI + KpnI: Double digest is not recommended. A sequential digest is required, using each enzyme in its supplied NEBuffer. Note: The above recommendation is based on the experimental results. Please check Suggested NEBuffers for Double Digestion. *??BamHI has a High Fidelity version BamHI-HF (R3136)????KpnI has a High Fidelity version KpnI-HF (R3142)????High Fidelity (HF) Restriction Enzymes have been engineered for reduced star activity????and have 100% activity in buffer 4 which may simplify your double digest. Double Digests Digesting a DNA substrate with two restriction endonucleases simultaneously (double digestion) is a common timesaving procedure. Selecting the best NEBuffer to provide reaction conditions that optimize enzyme activity as well as avoid star activity associated with some enzymes is an important consideration. Each enzyme is supplied with its optimal NEBuffer to ensure 100% activity. NEBuffer compositions are listed on buffer pages and on the data card sent with each enzyme. The Activity Chart for Restriction Enzymes rates the percentage activity of each restriction endonuclease in the four standard NEBuffers and NEBuffer EcoRI.Please use NEB Double Digest Finder to perform a double digest calculation. Suggested NEBuffers for Double Digestion Enzyme ? AatII AvrII BamHI BglII BsgI EagI EcoRI EcoRV HindIII KpnI MseI NcoI NdeI NheI NotI PstI PvuI SacI SacII SalI SmaI SpeI SphI XbaI XhoI XmaI NEBuffer 4 4 3 3 4 3 EcoRI 3 2 1 4 3 4 2 3 3 3 1 4 3 4 4 2 4 4 4 AatII 4 -- 4 seq seq 4 seq seq 4 4 4 4 4 4 4 seq 4 seq 4 4 seq 4 4 4 4 4 4 AvrII 4 4 -- 3 2 4 3 EcoRI 2 2 1 4 4 4 2 3 3 2 1 4 3 4 4 2 4 4 4 BamHI 3 seq 3 -- 3 3 3 EcoRI 3 seq seq 3 3 3 seq 3