H102对APP转基因小鼠突触相关蛋白表达的影响-天津医药.PDFVIP

天津医药圆园10 年5 月第38 卷第5 期 405 H102 对APP 转基因小鼠突触相关蛋白表达的影响* 吟 王 曼 徐淑梅 马志红 摘要 目的：观察H102 对APP 转基因小鼠脑内超微结构-突触后致密区（PSD-95 ）和突触素表达的影响。方法： 将16 只APP 转基因小鼠随机分为模型组和给药组，每组8 只。并设同月龄同背景C57BL/6J 小鼠为正常组。给药组侧 脑室注射 H102 生理盐水溶液，正常组和模型组侧脑室注射等体积生理盐水，3 滋L/d, 注射30 d 后行行为学检测即水 迷宫测试，然后取出并固定脑组织，利用免疫组化及宰藻泽贼藻r灶 遭造燥贼 方法测定小鼠脑组织突触素、孕杂阅原怨缘 及泽澡葬灶噪员 的表 达。结果：定位航行实验给药组APP 转基因小鼠逃避潜伏期从第 2 天较模型组差异有统计学意义（P 0.05 ），较正 常组从第 3 天差异无统计学意义（P 0.05 ）；空间探索实验第三象限停留时间和跨越平台次数较模型组差异有统计 意义（P 0.05 ），较正常组差异无统计学意义（P 0.05 ）。模型组皮质和海马出现突触素、PSD-95 及shank1 表达减少； 注射H102 后突触素、PSD-95 及shank1 表达较正常对照组差异无统计学意义（P 0.05 ）。结论：H102 对阿尔茨海默病 （AD ）治疗有一定的应用价值。 关键词 阿尔茨海默病 小鼠, 转基因 突触蛋白类 动物实验 小鼠，近交C57BL PSD-95 shank1 Effects of H102 on Synapse-Related Protein Synaptophysin of APP Transgenic Mice WANG Man, XU Shumei, MA Zhihong Department of Phys iology, Tianj in Medical Univ ers ity, Tianj in 300070, China To observe the effect of H102 on synapse-related protein synaptophysin in APP transgenic mice. Abstract Objective ： Methods: The APP transgenic mice were randomly divided into two groups, model group, H102 treatment group, and control group (C57BL/6J mice with the same age and background to model ones). Mice in model and normal control group were treated with saline solution, while mice of H102 treatment group were treated with compounds (80 滋mol/L袁 H102 in saline solution) inj ected into the lateral cerebral ventricle once a day for 30 days. Then Morris water maze test was performed. At the end of the experiments mice were killed and immunohistichemical stain and western blot were used to detect the synaptophy