丙酮固定骨髓活检gma塑料包埋.docVIP

丙酮固定骨髓活检GMA塑料包埋 切片免疫组化检测的研究 浦杰 莫东华 刘健 桂林医学院附院血液病科 广西桂林541001 摘要： 目的 为探讨丙酮固定骨髓GMA塑包切片内保留敏感造血分化抗原的新方法。 方法 使用冷丙酮固定结合简易三步塑包体系，观察了38例血液病毒者骨髓GMA包埋切片内14种单克隆标记物表达的研究结果。 结果 此简易三步塑胶包埋体系不仅能提供卓越的细胞形态，细胞抗原性也获较佳保存，并可在切片内进行C-MYC原癌基因表达的检测。 结论 本文建立了一种骨髓活检的简易塑包操作技术，既可获卓越的抗原性保留，也有助于对免疫组化标记物相关的形态学细节进行观察。 关键词：丙酮固定；骨髓活检；塑料包埋；免疫组织化学 Study of immunohistochemical detection with glycol-methacrylate(GMA) plastic cembedding section using acetone-fixed bone marrow biopsies. Pu Jie，Mo Dong hua，Liu Jian [Department of Hematology,The affiliated Hospital of GuiLin Medical college,GuiLin,541001,China] Abstract:objective The primary purpose of this study was to explove a new processing method that preserved Sensitive hematopoietic differentiation antigens with glycol-methacrylate(GMA) plastic embedding sections using acetone-fixed bone marrow. Method using cold ecetone-fixation with A simplified three-step plastic embedding system were observated in 38 patients with henatological disorders by glycol-methacrylate embedding Sections for bone marrow biopsies which results of study of 14 monoclonal markers and its expression. Results A simplified three-step plastic embedding system has been found to provide both excellent morphologic details and autigenic preservation.The descvibed here demonstrates that for C-myc expression in bone marrow biopsies sections. Conclusion We describe here a simplified plastic embedding technigue for bone marrow biopsies which allows achieve excellent autigence preservation and high quahity observation of morphological detals in relation to immunohistochemical markers. Key words：acetone fixation；bone marrow Biopsy；plastic embedding；immunohistochemistry 乙二醇－甲基丙烯酸酯（GMA）塑包剂于室温下聚合时不仅强烈放热，并可与蛋白质氨基终端位结合而遮蔽细胞表面抗原决定簇，阻抑抗原－抗体间反应，无法进行免疫表型检测，本文报道以冷丙酮固定结合简易三步塑包体系可有效进行骨髓切片免疫酶标染色的研究，适于在任何条件的血液病理实验室中推广应用。 1 资料和方法 1.1 病例 2008年2月－2011年6月共收集38例各种血液病患者；男21例，女17例；年龄15－82岁。计骨髓增生异常综合征（MDS）难治性贫血（RA）2例，伴多系病态造血的难治性血细胞减少症（RCMD）3例；急性髓细胞白血病（AML）M1 2 例、M2 4例、M3 2 例、M5 2例和M6 2例；急性淋巴细胞白血病（A