山药雌激素活性成份分析与研究studyofestrogenic-义守大学.pdfVIP

山藥雌激素活性成份分析與研究 Study of Estrogenic Compounds in Yam Tuber 鄭瑋宜 1 黃青真 2 1 2 義守大學醫學營養學系 台灣大學生化科技系 e-mail : wicheng@isu.edu.tw 摘 要 Abstract 婦女停經後因卵巢逐漸萎縮，分泌雌 Yam (Dioscorea spp.) is a common food 激素功能劇降，產生更年期症狀；長期更 in tropical area and has been shown to improve the status of sex hormone in 使骨質疏鬆且心血管疾病危險率提高。山 postmenopausal women. However, 藥 （ Dioscorea spp. ） 是 薯 蕷 科 phytoestrogenic activity and active （Dioscoreaceae ）、薯蕷屬（Dioscorea ） compound have never been reported. In this 多年生蔓性根莖類植物某些品系山藥， （如 study, estrogenic activity of yam was D. villosa ）製劑已在北美地區供為停經後 examined and active compounds were 婦女之膳食補充品，但功效不明。有研究 isolated and identified based on the 顯示，山藥能改善停經後婦女之生理狀 ligand-dependent transcriptional activation 況，暗示山藥可能含植物雌激素活性成 through estrogen receptors. Ethyl acetate 份。本實驗主要目的為建立一分子篩選模 extracts of various species/varieties of yam 式（transactivation assay ），用以篩選具雌 were found to activate estrogen receptors α and β to various extents. The extract of 激素活性之食材。分別選殖含有 ERα及 Dioscorea alata cv. Tainung No.2 tuber was ERβ LBD chimeric receptor質體，將其與 fractionated by repeated silica gel column ALP 表現基因