时间对edta依赖性血小板减少症患者血小板检测的影响-中国医药指引.docVIP

时间因素对EDTA-K2依赖性血小板减少症患者 血小板计数的影响 王园园1; 王保峰2; 付书贞11）河南省肿瘤医院450008 2河南省中医药研究院附属医院 郑州 450003 【摘要】探讨时间对依赖性血小板减少症（EDTA-PTCP）的。确诊为分别采集抗凝于、、SYSMEX XN-2000、手工法计数血小板，以及血涂片瑞氏染色观察血小板聚集情况。结果EDTA-K2抗凝在的结果(P0．05)，、0min、60min和120min的结果与手工计数值差异均有统计学意义(P均0．05)。结论 时间对EDTA依赖性血小板减少症患者血小板检测有影响，对于该类患者应完成，并同时进行镜检。 EDTA-PTCP； 血小板计数；血涂片 The influence of time factor on platelet count in EDTA-K2-dependent thrombocytopenia (EDTA-PTCP ) patient 【Abstract】time factor on platelet count in EDTA-K2-dependent thrombocytopenia (EDTA-PTCP ) patient. Methods Collect 39 patients in Henan Tumor Hospital that diagnosed EDTA-dependent thrombocytopenia during 2014 ~ 2012, collect EDTA-K2 anticoagulant at 0min、、latelet count or observing the aggregation situation: auto-blood cell analyzer SYSMEX XN-2000, manual method, and blood smear. Results The platelet count in 0min was closest to manually count result, the difference was not statistically significant (P 0.05), while in 10min, 30min, 60min and 120min of the test, differences were statistically significant (P 0.05). Those blood samples could be found the platelet count normal, as time progressed, as time progresses, scattered platelets gradually become less, piles of platelet gradually increased. Conclusion Time factor is influential on platelet count in EDTA-PTCP patients, for these patients, detection should be completed as soon as possible on the machine, and the blood smear should be made and be observed on microscope at the same time for the accuracy of the results. 【Key words】time; EDTA-PTCP; latelet count; blood smear 血细胞计数是临床常规检验项目，血小板是其中重要的指标之一，结果准确直接影响患者的诊断与治疗。乙二胺四乙酸盐（ EDTA）是血细胞计数常用的抗凝剂，但可引起血小板聚集，造成血小板计数减低，称为EDTA依赖性假性血小板减少症（ EDTA-PTCP ），给临床诊治过程带来很大困扰1 材料与方法 1.1 材料 收集河南省肿瘤医院2012~2014年期间确诊为EDTA-PTCP的患者39例日本SYSMEX山东奥赛特公司抽取EDTA抗凝血标本，分别于送检后的0min、10min、30min60min和120min在仪器上进行检测，并同时改良牛鲍计数板手工计数和推片染色镜检，血小板数量随时间变化的趋势和聚集程度。 1.4 统计方法 使用SPSS 19．O统计软件进行处理，计量资料以均数士标准差（x ± s）表示，EDTA‐K2 抗凝结果采用 t检验，P为差异有统计学意义。、0min、10min、30min60min和120min之间分别进行比较，差异均无统计学意义。说