沙棘叶中多糖的初步研究多杰才旦.docVIP

沙 棘 叶 中 多 糖 的 初 步 研 究 分类： 毕业论文 20006 摘 要 本实验首次探讨了沙棘叶中多糖的提取分离，测定其多糖的百分含量。 首先用蒸馏水清洗干叶，以洗掉尘土、浮渣，去掉粗梗，剪细。适量石油醚反复脱脂，滤渣自然挥干，再用常温水浸泡一次，80℃热水浸泡两次，以提取水溶性物质，然后合并所有水提液，采用旋转蒸发仪在适宜条件（t＝50℃，抽真空0.09Mpa）下浓缩溶液至适宜体积，sevage法反复操作去蛋白，最后用95％的乙醇使溶液达到不同的浓度而沉淀出多糖，优选出沉淀沙棘多糖的最优醇浓度。0－5℃冰箱中放置48小时后，过滤时用无水乙醇，丙酮洗数次，真空抽干。 粗产品重新溶解于适量水中，然后通过大孔树脂进行纯化，蒸馏水洗脱，洗脱液在PH值为8时，用20％的H2O2，50℃以下保温2h脱色，以保证产品的颜色，最后用95％的乙醇使溶液醇浓度达到80％以析出多糖，重复上面的析糖操作，最后保存产品于干燥处。 用D（＋）—葡萄糖作标准，苯酚－硫酸法制备标准曲线，求出线性回归方程，从而得到粗多糖的含量，由此也可以算出沙棘叶中多糖的百分含量。 然后进行TLC法初步定性确定多糖成分，再进行核磁共振定性分析，即可检验其准确成分。 关键词 沙棘；多糖；提取；脱蛋白；AB－8大孔树脂 ? Abstract The experiment is the first to research the Hippohae rhamnoides L leaves’ polysaccharides .The popose of the experiment is taking the polysaccharides from the Hippohae rhamnoides L leaves. First wash the leaves through the water to clean the dirt away, then use petroleum ether to degrease the leaves many times, extract the water-soluble material through hot water of 80℃, utilizing the revolval-evaporation under the condition( t=50 ℃, vacuum 0.09 Mpa) to concentrate the aqua to the proper physical volume . Isolation and composition of polysaccharides from the aqua ,alcohol precipitate by hot water extraction and remove protein from polysaccharides by sevag,in order to get optimum alcohol concentration from precipitating Hippohae rhamnoides L polysaccharides. And also try polysaccharides purified by AB-8 Macfofeticulaf Resin to achieve good results.Finally sum up the optimum technology of extraction and purification polysaccharides from the leaves. then lying in the refrigerator(0-5℃)two days,fitrated by alcohol , acetone,more than three times . Dissolve into the proper water again;through AB－8 Macfofeticulaf Resin,water cleanse; add the sodium hydroxide to make the water’s PH 8 ,use the hydrogen peroxide (20% ) ,below 50 ℃ preservation 2 hours to ensure the color of the products.;then alcohol precipitating again. With the D(+)- the glucose as the standard to figure out the standard curve in the way o