奈米电浆子感测技术於生物分子之功能分析.PDFVIP

快、準、多樣之 生醫感測術專題 奈米電漿子感測技術於生物分子之功 能分析 Nanoplasmonic Sensing for Biomolecular Function Analysis 簡汎清、邱國智、林俊佑、易政男、陳顯禎 Fan-Ching Chien, Kuo-Chih Chiu, Chun-Yu Lin, Jenq-Nan Yih, Shean-Jen Chen 表面電漿子共振生物感測技術對偵測表面環境變化具有高靈敏度且不需外加任何標記等優 勢，已廣泛應用於生物分子交互作用分析上。然而傳統表面電漿子共振生物感測技術仍難以 直接偵測極微小分子量或極低濃度下分子間交互作用，且表面電漿子共振可提供生物分子交 互作用分析之動力學資訊，但完整的生物分子辨識系統更應具有偵測構形與結構改變之能 力。本研究藉由嶄新的奈米電漿子技術來解決上述三個關鍵問題。首先，利用圖樣化金奈米 粒子來操控粒子電漿子或稱局域表面電漿子以提升感測器之靈敏度約十倍；再者，研發出耦 合波導表面電漿子共振生物感測器，可直接即時地監測蛋白分子構形變化；最後，結合衰減 全反射之表面強化拉曼散射以提供生物分子結構資訊。利用這些奈米電漿子技術，可協助快 速診斷、藥物研發與蛋白質體學等研究建立一個嶄新的生物分子功能分析平台。 Surface plasmon resonance (SPR) biosensing has become a standard practice in the investigation of biomolecular interaction analysis (BIA), because it is highly sensitive to the resonance condition on the sensing surface caused by environmental changes and do not require any extrinsic labeling. However, the detection limit of the conventional SPR biosensors is insufficient for the monitoring of low concentrations of small biomolecular analytes. In addition, the conventional SPR biosensor only can provide kinetic analysis information in the BIA. A more powerful biorecognition system is required not only to provide the kinetic analysis, but also to have the capability of monitoring biomolecular conformational and structural changes or trends. Therefore, in this study, nanoplasmonic technology was used to overcome three above challenges. First, patternized gold