样本处理（Sample processing）.docVIP

样本处理（Sample processing） ELISA samples were collected and pre prepared The clinical test of common use ELISA sample generally include blood (refers to blood, blood, urine, stool), cerebrospinal fluid, pleural fluid, prostatic fluid, semen, vaginal secretions, have certain requirements of these sample collection time, and preservation method. (1) collection of clinical samples A, blood samples Some physiological factors, such as smoking, eating, exercise, mood swings, pregnancy and body position, can affect the changes in certain components of the blood, some even changing day and night. Therefore, the sampling of blood specimens should be disturbed as much as possible, and the condition should be consistent. If it can not be avoided, the factor should be indicated on the specimen. 1. peripheral blood Generally choose the left hand ring finger medial blood sampling, the site should be no frostbite, inflammation, edema, damage. If this part does not meet the requirement, replace it with other fingers. For patients with burns, you can choose to take blood from the whole skin. As a result of some conventional blood tests (such as white blood cell count, classification, etc.), the physiological factors are too large to fluctuate, so that the conditions should be consistent as much as possible. The project involves the detection of coagulation function in vivo, (such as platelet counts, bleeding time or coagulation time) detection, we must pay attention to whether the patient had anticoagulant and procoagulant drugs, in order to reduce or avoid the interference factors. 2. venous blood In addition to all kinds of hemostasis and thrombus testing, the anticoagulant venous blood and plasma should be used. At present, the majority of the testing items can be directly analyzed by using the serum of venous blood. The serum test project, some (such as blood glucose, blood lipid) affected by diet and circadian factors, usually in the early morning fasting blood samples as appropri