方法湖泊沉积计年研究新进展.PDFVIP

中国农业科学 2006,39(4):709-714 Scientia Agricultura Sinica 稻瘟菌Cdc42若干推测互作蛋白的结构和表达特点 郑 武，陈继圣，郑士琴，鲁国东，王宗华 （福建农林大学生物农药与化学生物学教育部重点实验室，福建农林大学功能基因组学研究中心，福州 350002 ） 摘要：【目的】已有研究表明稻瘟菌Cdc42(MgCdc42)与酵母Cdc42(ScCdc42)高度同源，参与调控其形态分化 和侵染过程，通过分析可能的 MgCdc42 互作蛋白，以明确这些蛋白结构和功能。【方法】用 ScCdc42 互作蛋白经 BLAST 有哪些信誉好的足球投注网站获得了稻瘟菌基因组中的相应同源物，分析了这些同源物的结构，并通过半定量 RT-PCR 分析 MgCdc42 不同突变情况下这些可能的调控蛋白及效应蛋白的表达情况。【结果】MgCdc42正显性突变后，导致所有推测互作 蛋白表达量均有所提高；MgCdc42负显性突变，除MgBem1、Chm1、MgGic1 表达量未见明显变化外，其余表达量均 有所降低；当MgCdc42失活后，所有可能的MgCdc42调控蛋白及效应蛋白之表达量均有所降低。【结论】稻瘟菌可 能存在酵母Cdc42相似的信号途径，MgCdc42在其中起着重要的调控作用。 关键词：Cdc42；稻瘟菌；鸟苷酸交换因子；GTP酶激活蛋白 Structure and Expression Pattern of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea ZHENG Wu, CHEN Ji-sheng, ZHENG Shi-qin, LU Guo-dong, WANG Zong-hua （The Ministry of Education Key Laboratory of Bio-pesticide and Chemistry Biology, Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002) Abstract: 【Objective 】 MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in its morphogenesis and infection process. To further dissect the signaling network, the putative MgCdc42-interacting proteins were analyzed. 【Method 】 ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semi-quantitative RT-PCR. 【Result 】All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expr