黄芪减轻血管紧张素II诱导的小鼠血管重塑-第三军医大学学报.DOC

黄芪对血管紧张素II诱导的小鼠血管重塑的保护作用 裴芳1, 2 王新全2,岳荣川2,黄骥1,黄婕1,刘筑3,伊远学3,王旭开2,于长青2 400061重庆,武警重庆总队医院心血管内科1；400042重庆,第三军医大学大坪医院野战外科研究所心血管内科,重庆市心血管病研究所2；400061重庆, 武警重庆总队医院科教中心3） [ 摘要目的 探讨黄芪对血管紧张素II诱导的血管重塑的作用及其可能机制。方法 将40只雄性C57BL/6小鼠随机分为对照组、血管紧张素II（AngII）处理组、Ang II+?Losartan?治疗组及Ang II+黄芪治疗组，每组各10只。对照组给予生理盐水0.2 mL/d灌胃，其余3组均采用皮下埋微渗透泵方法，以200 ng/（kg·min）的量缓慢释放Ang II建立血管重塑模型。其中Losartan治疗组以10 mg/（kg·d）剂量灌胃，黄芪治疗组以20 g/（kg·d）剂量灌胃。所有小鼠总计处理14 d。每2 d以尾套法测定收缩压。第14 d时处死小鼠，收集主动脉进行HE及Masson染色检测血管重塑情况。RT-PCR检测主动脉I型胶原转录水平表达。Western Blot检测血管紧张素转换酶2（ACE2）及Ang II 1型受体（AT1R）的蛋白水平表达。结果 黄芪处理可以显著降低Ang II引起的血压升高（P0.05）。黄芪处理可对抗Ang II引起的主动脉管壁增厚和纤维化增加、降低I型胶原mRNA表达（P0.05）、上调ACE2蛋白表达（P0.05）并下调AT1R蛋白表达（P0.05）。结论 黄芪可减轻Ang II诱导的血管重塑，其机制可能是通过上调ACE2和下调AT1R。关键词：血管紧张素II；血管重塑；纤维化；黄芪 中图分类号：R543.5 文献标志码：A Astragalus membranaceus attenuates angiotensin II induced vascular remodeling in mice Pei Fang 1,2, Huang Ji 1, Huang Jie 1, Wang Xinquan 2, Yue Rongchuan 2, Liu Zhu 3, Yi Yuanxue 3, Wang Xukai 2, Yu Changqing 2 (1Department of Cardiology, Chongqing Corps Hospital of Chinese People′s Armed Police Force, Chongqing, 400061,China; 2Department of Cardiology, Chongqing Institute of Cardiovascular Diseases, Institution of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China; 3The Center of scientific Research, Chongqing Corps Hospital of Chinese People′s Armed Police Forces, Chongqing, 400061, China) [Abstract] Objective To investigate the effect of astragalus membranaceus on angiotensin II (AngII) induced vascular remodeling in mice and its underlying mechanism. Methods A total of 40 male C57BL/6 mice were randomly divided into 4 groups (n=10/group) including control group, AngII group, AngII+Losartan group and AngII+Astragalus group. In control group, mice were given 0.2 ml/d normal saline by gastric gavage. In the other 3 groups, mice were given 200 ng/(kg·min) AngII by an osmotic pump embedded subcutaneously. In addition, in AngII+Losartan group, mice were given 10 mg/(kg