不同状态下家兔Oddi括约肌肌电活动实验模型建立.docVIP

不同状态下家兔Oddi括约肌肌电活动实验模型建立 　 作者：徐红梅，李虎城，胡志安，赵红梅，董家鸿 【关键词】 状态 　　[摘 要]目的建立不同状态下家兔Oddi括约肌(sphincter of Oddi, SO)肌电活动实验模型。方法 利用电生理方法记录家兔Oddi括约肌的肌电活动，并建立空腹、进食、药物兴奋和药物抑制等四种状态下Oddi括约肌肌电活动的实验模型。结果空腹状态下家兔SO肌电表现为规律的、单发性的锋电位(spike potentials of SO, SPSO)；禁食18 h后，给予50 ml牛奶，SO肌电表现为规律的、间断的肌电簇(myoelectronic activity of SO, MASO)；静脉注射1 mg吗啡和2 mg新斯的明后，SO肌电活动首先表现为数个长时间、不间断的SPSO组成的肌电串，120～150 min内完全抑制，抑制期过后又恢复单个SPSO；静脉给予山莨菪硷1 mg，SPSO随即消失。结论 成功建立了四种状态下家兔Oddi括约肌肌电活动实验模型。 　　 [关键词] Oddi括约肌；肌电活动；家兔 　　Establishment of rabbit model for the research on myoelectric activities of sphincter of Oddi under different conditions 　　Abstract: Objective To establish rabbit models for the study on myoelectric activities of sphincter of Oddi (SO) under different conditions. Methods Myoelectric activities of SO were recorded by using electrophysiological methods under four different conditions, including fasting for 18 h, feeding, druginduced excitatory state and druginduced inhibitory state. Results Single and regular spike potentials of SO (SPSO) were observed after fasting for 18 h. Regular and discontinuous myoelectronic activity of SO (MASO) was found after intaking 50 ml milk. Longterm and continuous SPSO were immediately observed after intravenous infusion of 1 mg morphine and 2 mg neostigmine, and then the myoelectric activities of SO were significantly blocked in 120-150 min. Finally, the myoelectric activities recovered to the control levels. SPSO disappeared at once after intravenous injection of 1 mg anisodamine. Conclusion Rabbit models for the study on myoelectric activities of SO under different conditions have been established successfully. 　Keywords: sphincter of Oddi; myoelectric activity; rabbit 　　Oddi括约肌（sphincter of Oddi，SO）位于十二指肠乳头周围，由胆总管括约肌、胰管括约肌以及壶腹部括约肌构成。SO被喻为调节胆汁和胰液流动的“闸门”，同时还有防止十二指肠内容物反流入胰胆系统等功能。多种胆道和胰腺疾病均与SO的功能障碍密切相关。因此，SO运动功能的研究是胰胆系统生理和病理生理领域的重要内容[1]。为了探索SO的运动规律和特点，本实验以家兔为研究对象，记录其SO的肌电活动信号，并建立不同状态下SO肌电活动的实验模型，从电生理的角度进一步探明SO的活动规律。 　　1 材料与方法 　　1.1实验动物及分组 　　健康成年家兔32只，由第三军医大学实验动