PTEN、P27在子宫内膜癌中表达及其临床意义.docVIP

PTEN、P27在子宫内膜癌中表达及其临床意义 　【摘要】 目的：探讨抑癌基因PTEN与细胞周期调控因子P27在子宫内膜癌中的表达，探讨其临床意义。方法：采用免疫组化法对正常子宫内膜、子宫内膜增生过长、子宫内膜不典型增生各20例，子宫内膜癌50例及子宫内膜癌旁组织50例中PTEN及P27的表达进行检测。结果：PTEN在正常子宫内膜、子宫内膜增生过长、子宫内膜不典型增生、子宫内膜癌旁组织及子宫内膜癌中阳性表达率逐渐下降，P27在子宫内膜癌中阳性率为34%。PTEN、P27皆与组织学分级有关（P＜0.01）。结论：PTEN基因失活和P27蛋白缺乏参与了子宫内膜癌的发生、发展，而且与癌细胞的分化有关。 【关键词】 子宫内膜癌； PTEN； P27 [Abstract] Objective：To study the expression and its clinical significance of PTEN and P27 in the pathogensis of endometrial carcinoma. Methods: A specific and sensitive immunohistoche mical S-P assay was used to detect the expressions of PTEN and P27 in paraffin-embedded sections of 20 cases of normal endometrium，endometrial hyperplasia and atypical hyperplasia of endometrium, and 50 samples of endometrial carcinoma and its nearby tissue respectively. Results：The positive rates of PTEN in normal proliferative endometrium , endometrial hyperplasia , atypical endometrial hyperplasia , endometrial carcinoma and its adjacent tissue were gradually lowered . The difference was significant . The positive rate of the aberrant expressions of P27 was 34%.In endometrial carcinoma the positive rate of PTEN or P27 expression was significantly related to the cell grade（P＜0.01）. The difference was significant. Conclusions: Inactivation of PTEN and absence of P27 may play some roles in the carcinogenesis and development of endometrial carcinoma and its cell differentiation. [Key words] Endometrial carcinoma；Phosphatase and tensin homology deleted on chromosome ten；P27 子宫内膜癌近年发生率呈上升趋势，尽管其早期诊断和预后相对较好，但是它的发生的分子机制尚不清楚。PTEN（phosphatase and tensin homology deleted on chromosome ten）是近年来发现的具有双重特异性磷酸酶活性的抑癌基因[1]。P27是细胞周期调控核心机制—细胞周期素（cyclins）—细胞周期素依赖性蛋白激酶（CDKs）—细胞周期素依赖性蛋白激酶抑制剂（CDKIs）网络调控系统中CDKIs中的一员[2]。本文采用免疫组化法，对我院1996年1月~2005年12月收治的50例子宫内膜癌进行PTEN和P27检测，探讨其在子宫内膜癌中的发生、发展中的作用和临床意义。 1 材料与方法 1．1 临床资料 子宫内膜癌患者50例，年龄21～85岁，平均56.25岁，选取同期诊刮后正常子宫内膜、子宫内膜增生过长、子宫内膜不典型增生各20例（子宫内膜癌旁组织50例），子宫内膜癌患者均行广泛或次广泛子宫切除加盆腔淋巴结清扫，术前未经任何治疗，病例均经病理证实。 1．2 检测方法 子宫内膜癌及相应癌旁组织、正常子宫内膜、子宫内膜增生过长、子宫内膜不典