PTEN、VEGF-C及VEGFR-3在胃癌组织中表达及临床意义.docVIP

PTEN、VEGF-C及VEGFR-3在胃癌组织中表达及临床意义 　【摘要】 目的：研究PTEN基因（phosphatase and tensinhomolog deleted on chromosome ten）、血管内皮生长因子-C（vascular endotnelial growth factor C, VEGF-C）和血管内皮生长因子受体-3（vascular endotnelial growth factor receptor 3, VEGFR-3）在胃癌及正常胃组织中的表达，探讨其在胃癌的发生、发展、浸润和转移中的作用。方法：选择临床病理资料齐全的胃癌蜡块标本54例，采用免疫组织化学SP法检测PTEN、VEGF-C和VEGFR-3在其中的表达。另取正常胃黏膜标本15例为对照组。采用χ2检验和相关分析。结果：胃癌组织中PTEN低表达，且在肿瘤浸润深、有淋巴转移、临床分期高、病理分化低时降低；VEGF-C高表达，且在肿瘤直径大、肿瘤浸润深、有淋巴转移、临床分期高、病理分化低时增高；VEGFR-3高表达，且肿瘤直径大、肿瘤浸润深、有淋巴转移、临床分期高时升高。胃癌组织中VEGF-C与VEGFR-3的表达呈正相关，PTEN与VEGF-C、VEGFR-3的表达均呈负相关（P＜0.05）。结论：检测PTEN、VEGF-C和VEGFR-3在胃癌组织中的表达情况对胃癌的诊断、预防和预后判断有指导意义。 【关键词】 胃肿瘤 PTEN 血管内皮生长因子 免疫组织化学 【ABSTRACT】 Objective: To Study the expression of PTEN, VEGF-C and VEGFR-3 in gastric cancer and normal gastric tissues. Methods: Fifty-four cases of gastric carcinoma specimens were selected with full clinicopathological data, and another 15 cases of normal gastric mucosa specimens were selected as the control. S-P immunohistochemistry was used to measure the ex pression of PTEN, VEGF-C and VEGFR-3. The data were statistically analyzed by χ2 test and relative analysis. Results: The expression of PTEN in gastric cancers was low and reduced with increased invasive depth clinical stages, histological differentiation and occurring of lymphatic metastasis. The expression of VEGF-C in gastric cancers was high and increased with increased diameter of tumor, invasive depth, clinical stages histological differentiation, and occurring of lymphatic metastasis. The expression of VEGFR-3 in gastric cancers was high, and increased with increased diameter of tumor, invasive depth and clinical stages, and occurring of lymphatic metastasis. The expression of VEGF-C and VEGFR-3 were of positive correlation, and PTEN and VEGF-C, PTEN and VEGFR-3 were of inversely correlation in gastric cancers. Conclusion: The expression of PTEN, VEGF-C and VEGFR-3 in gastric caners might be useful to estimate the prevention, diagnoses, therapy and prognosis of gastric c