detection of mycosphaerella graminicola in wheat leaves by a microsatellite dinucleotide specific-primer由微卫星检测球腔菌属graminicola小麦叶子二核苷酸specific-primer.pdfVIP
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detection of mycosphaerella graminicola in wheat leaves by a microsatellite dinucleotide specific-primer由微卫星检测球腔菌属graminicola小麦叶子二核苷酸specific-primer
Int. J. Mol. Sci. 2011, 12, 682-693; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Detection of Mycosphaerella graminicola in Wheat Leaves by a Microsatellite Dinucleotide Specific-Primer Kamel Abd-Elsalam 1,2,3,*, Ali H. Bahkali 2, Mohamed Moslem 2, Pierre J. G. M. De Wit 2,4 and 1 Joseph-Alexander Verreet 1 Institute of Phytopathology, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 9, D-24118, Kiel, Germany; E-Mail: javerreet@phytomed.uni-kiel.de 2 Botany and Microbiology Department, College of Science, King Saud University, P.O. Box: 2455, Riyadh 1145, Saudi Arabia; E-Mails: abhakali@.sa (A.H.B.); mbmoslem@.sa (M.M.) 3 Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt 4 Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB Wageningen, The Netherlands; E-Mail: pierre.dewit@wur.nl * Author to whom correspondence should be addressed; E-Mail: kamel200@.sa; Tel.: +966-92-1467-580; Fax: +966-1467-5833. Received: 2 December 2010; in revised form: 4 January 2011 / Accepted: 4 January 2011 / Published: 19 January 2011 Abstract: Early detection of infection is very important for efficient management of Mycosphaerella graminicola leaf blotch. To monitor and quantify the occurrence of this fungus during the growing season, a diagnostic method ba
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