detection of inferred ccr5- and cxcr4-using hiv-1 variants and evolutionary intermediates using ultra-deep pyrosequencing检测推断ccr5和cxcr4-using hiv - 1的变异和进化中间体使用超深焦磷酸测序.pdfVIP

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2017-09-12 发布于上海
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detection of inferred ccr5- and cxcr4-using hiv-1 variants and evolutionary intermediates using ultra-deep pyrosequencing检测推断ccr5和cxcr4-using hiv - 1的变异和进化中间体使用超深焦磷酸测序.pdf

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detection of inferred ccr5- and cxcr4-using hiv-1 variants and evolutionary intermediates using ultra-deep pyrosequencing检测推断ccr5和cxcr4-using hiv - 1的变异和进化中间体使用超深焦磷酸测序

Detection of Inferred CCR5- and CXCR4-Using HIV-1 Variants and Evolutionary Intermediates Using Ultra-Deep Pyrosequencing 1 2. 1.¤ 3 2 Evelien M. Bunnik , Luke C. Swenson , Diana Edo-Matas , Wei Huang , Winnie Dong , Arne 3 3 3 1¤ 2 Frantzell , Christos J. Petropoulos , Eoin Coakley , Hanneke Schuitemaker , P. Richard Harrigan , ´ 1¤* Angelique B. van ’t Wout 1 Department of Experimental Immunology, Sanquin Research, Landsteiner Laboratory, Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, 2 British Columbia Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada, 3 Monogram Biosciences, South San Francisco, California, United States of America Abstract The emergence of CXCR4-using human immunodeficiency virus type 1 (HIV-1) variants is associated with accelerated disease progression. CXCR4-using variants are believed to evolve from CCR5-using variants, but due to the extremely low frequency at which transitional intermediate variants are often present, the kinetics and mutational pathways involved in this process have been difficult to study and are therefore poorly understood. Here, we used ultra-deep sequencing of the V3 loop of the viral envelope in combination with the V3-based coreceptor prediction tools PSSMNSI/SI and geno2pheno[coreceptor] to detect HIV-1 variants during the transition from CCR5- to CXCR4-usage. We analyzed PBMC and