detection of mycobacterium tuberculosis in sputum by gas chromatography-mass spectrometry of methyl mycocerosates released by thermochemolysis由气相色谱分析-质谱法检测结核分枝杆菌的痰甲基mycocerosates thermochemolysis发布的.pdfVIP

Detection of Mycobacterium tuberculosis in Sputum by Gas Chromatography-Mass Spectrometry of Methyl Mycocerosates Released by Thermochemolysis 1¤ 2 3 3 4 Denise M. O’Sullivan , Simona C. Nicoara , Reggie Mutetwa , Stanley Mungofa , Oona Y-C. Lee , 4 2 1,3 1 2 David E. Minnikin , Max W. Bardwell , Elizabeth L. Corbett , Ruth McNerney *, Geraint H. Morgan 1 Department of Pathogen Molecular Biology, London School of Hygiene Tropical Medicine, London, United Kingdom, 2 Department of Physical Sciences, The Open University, Milton Keynes, United Kingdom, 3 Biomedical Research and Training Institute, Harare, Zimbabwe, 4 School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom Abstract Tuberculosis requires rapid diagnosis to prevent further transmission and allow prompt administration of treatment. Current methods for diagnosing pulmonary tuberculosis lack sensitivity are expensive or are extremely slow. The identification of lipids using gas chromatography- electron impact mass spectrometry (GC-EI/MS) could provide an alternative solution. We have studied mycocerosic acid components of the phthiocerol dimycocerosate (PDIM) family of lipids using thermochemolysis GC-EI/MS. To facilitate use of the technology in a routine diagnostic laboratory a simple extraction procedure was employed where PDIMs were extracted from sputum using petroleum ether, a solvent of low polarity. We also investigated a method using methanolic tetramethylammonium hydroxide, which facilitates d